Protein Info for ABIE53_003662 in Paraburkholderia graminis OAS925

Annotation: glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 250 to 267 (18 residues), see Phobius details TIGR01035: glutamyl-tRNA reductase" amino acids 4 to 413 (410 residues), 417.8 bits, see alignment E=2.5e-129 PF05201: GlutR_N" amino acids 6 to 161 (156 residues), 182 bits, see alignment E=1.2e-57 PF01488: Shikimate_DH" amino acids 177 to 310 (134 residues), 154 bits, see alignment E=5.3e-49 PF00745: GlutR_dimer" amino acids 325 to 415 (91 residues), 91.4 bits, see alignment E=8.1e-30

Best Hits

Swiss-Prot: 96% identical to HEM1_PARXL: Glutamyl-tRNA reductase (hemA) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 98% identity to bug:BC1001_3160)

MetaCyc: 46% identical to glutamyl-tRNA reductase (Escherichia coli K-12 substr. MG1655)
Glutamyl-tRNA reductase. [EC: 1.2.1.70]

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>ABIE53_003662 glutamyl-tRNA reductase (Paraburkholderia graminis OAS925)
MQLLTIGINHHTAPVALRERVAFPLEQIKPALDTFKSVWLGGTARSAPEAAILSTCNRTE
LYCATDDQAAREAAIQWLSRYHNLPIEELAPHVYALPQSEAVRHAFRVASGLDSMVLGET
QIVGQMKDAVRTASEAGALGTYLNQLFQRTFAVAKEVRSTTEIGAQSVSMAAAAVRLAQR
IFDKVSNQRVLFIGAGEMIELCATHFAAQQPRELVVANRTAERGTRLAERFNGRAIPLSD
LPTRMHEFDIIVSCTASTLPIIGLGAVERAVKARRHRPIFMVDLAVPRDIEPEVGKLEDV
FLYTVDDLGAIVREGNASRQAAVAQAEAIIETRVQNFMQWLEARSIVPVIRHMHTQADVL
RRAEVERAQKMLARGDDPAAVIEALSQALTNKLIHGPTHALNRASGENRDTLIELMGGFY
KHSGSSER