Protein Info for ABIE53_003602 in Paraburkholderia graminis OAS925

Annotation: glutamate N-acetyltransferase/amino-acid N-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 18 to 413 (396 residues), 396.9 bits, E=4.6e-123
• PF01960: ArgJ
  • amino acids 24 to 413 (390 residues), 474.1 bits, E=1.3e-146

Best Hits

Swiss-Prot: 91% identical to ARGJ_BURL3: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 98% identity to bug:BC1001_3099)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.1

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (413 amino acids)

>ABIE53_003602 glutamate N-acetyltransferase/amino-acid N-acetyltransferase (Paraburkholderia graminis OAS925)
MAVNFPSIDPAQLHPVAGVTLGWAEANIRKPNRKDVLVISVDEGATVGGVFTQNRFCAAP
VTVCRENLERVRAGGKPIRALVINTGNANAGTGEPGLAHARETCAELARLADIAPEQVLP
FSTGVILEPLPVDRLKAGLPAALANRKEANWYDAAQAIMTTDTLPKATSRQVTIEGHTVT
LTGISKGAGMIKPNMATMLGFLAFDAALAQPVLDALVRHVADRSFNCITIDGDTSTNDSF
ILIASGKSSLPAITSTDSPAYAALRDAVTEVAQTLAQLIVRDGEGATKFMTVQVEGGSSV
GECRQIAYAIGHSPLVKTAFYASDPNLGRILAAIGYAGIDDLDVGKIDLYLDDVLVATAG
GRNPAYREEDGQRVMKKSEIGIRVVLGRGNAQATIWTCDLSHDYVSINADYRS