Protein Info for ABIE53_003401 in Paraburkholderia graminis OAS925

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 288 TIGR02821: S-formylglutathione hydrolase" amino acids 3 to 286 (284 residues), 465.9 bits, see alignment E=2.4e-144 PF00756: Esterase" amino acids 22 to 280 (259 residues), 184.2 bits, see alignment E=3.7e-58 PF00326: Peptidase_S9" amino acids 138 to 269 (132 residues), 29.3 bits, see alignment E=6.1e-11

Best Hits

Swiss-Prot: 55% identical to ESTD_RAT: S-formylglutathione hydrolase (Esd) from Rattus norvegicus

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 93% identity to bgf:BC1003_2836)

MetaCyc: 49% identical to S-formylglutathione hydrolase FrmB (Escherichia coli K-12 substr. MG1655)
S-formylglutathione hydrolase. [EC: 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (288 amino acids)

>ABIE53_003401 S-formylglutathione hydrolase (Paraburkholderia graminis OAS925)
MLELLSSHACHGGEQRFYRHESHTIGLPMRFSVYLPPQALQASQANANAKVPALFYLAGL
TCTEETFAIKAGAQRFAAQHGVALVAPDTSPRGAGIPGESAAWDFGVGAGFYVDATQEPW
ARHYRMYSYVRDELRETVLNELPVDASRLGIFGHSMGGHGALMLALRNPDIYRSVSAFAP
IAAPTRCPWGEKAFTGYLGDDREAWKEYDASELVARASSKFAEGILLDQGLADQFLAEQL
YPDVFEAACRDAGQPLTLRRHAGYDHGYYFISTFIEDHIAHHAKVLLG