Protein Info for ABIE53_003172 in Paraburkholderia graminis OAS925

Annotation: 3-(3-hydroxy-phenyl)propionate hydroxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01494: FAD_binding_3" amino acids 10 to 363 (354 residues), 284.1 bits, see alignment E=2.6e-88

Best Hits

KEGG orthology group: None (inferred from 92% identity to bgf:BC1003_2623)

Predicted SEED Role

"Pentachlorophenol 4-monooxygenase (EC 1.14.13.50)" (EC 1.14.13.50)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.50

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>ABIE53_003172 3-(3-hydroxy-phenyl)propionate hydroxylase (Paraburkholderia graminis OAS925)
MNAAANNASPVLIVGAGPTGLAAAMSLARAHIPVRLIDRAMQPNPYSRAIGIQARTLELL
EQHRLAERFLELGHRARVANLFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHVA
TLGVTIERGVELTMFSQGSVGVQATLRRADGHTETLRPSYMIAADGAHSAIRHGLGLNFV
GKTSEQTFLLADVHAETDWPDDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPTPAAAA
PLSPVVLGEPPLARVRAPSLDECKALVARRVHERVDVSEVAWSSYFHVNSRMVERLRVGR
VFLAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARVLKGAAPDRLLDTYHIERHPIERDV
LRQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARLTISELSIQYRRSPLTLERV
LDGGPRAGERAPDAVVHVVDGPLGRAPGIGCIYDLHDPAFFSLFLLVPPLPADDKPLDPD
AKHAPPADAELERLTADMEKLLEGAVRIWRVTDTSGDGGPSLAESYGRTRPSFYLLRPDG
YISARGRTGSDLNGLLRHCEAWFTVDGQIPEQTAV