Protein Info for ABIE53_003170 in Paraburkholderia graminis OAS925

Annotation: formate dehydrogenase major subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 992 PF13510: Fer2_4" amino acids 59 to 133 (75 residues), 88.2 bits, see alignment 1.2e-28 PF10588: NADH-G_4Fe-4S_3" amino acids 140 to 179 (40 residues), 59.2 bits, see alignment (E = 8.8e-20) PF13237: Fer4_10" amino acids 201 to 260 (60 residues), 25.2 bits, see alignment 5.3e-09 PF12838: Fer4_7" amino acids 205 to 262 (58 residues), 31.4 bits, see alignment 8.5e-11 PF00037: Fer4" amino acids 246 to 262 (17 residues), 22.2 bits, see alignment (E = 3.8e-08) PF04879: Molybdop_Fe4S4" amino acids 278 to 328 (51 residues), 56.5 bits, see alignment 8.8e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 281 to 956 (676 residues), 936.1 bits, see alignment E=4.9e-286 PF00384: Molybdopterin" amino acids 333 to 750 (418 residues), 242.3 bits, see alignment E=3.2e-75 PF01568: Molydop_binding" amino acids 848 to 953 (106 residues), 105.4 bits, see alignment E=6.8e-34

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 94% identity to bpy:Bphyt_3023)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (992 amino acids)

>ABIE53_003170 formate dehydrogenase major subunit (Paraburkholderia graminis OAS925)
MSDMLNSTAGAPKQVPSAGGCGSGNCACKSQAQAQTQAPRSYFDETDFGTPARHADVDVT
LEIDGQAVTVPAGTSVMRAAVEAGVNVPKLCATDSLEPFGSCRLCLVEIEGKRGYPASCT
TPVEAGMKVRTQTDRLQSLRRNVMELYISDHPLDCLTCPANGDCELQDMAGVTGLREVRY
GFDGANHLNDKKDESNPYFTYDPAKCIVCNRCVRACEETQGTFALTIAGRGFESRVAASE
NQPFMDSECVSCGACVAACPTATLQEKTVIMLGQAEHSAVTTCAYCGVGCSFKAEMKGNQ
VVRMVPFKNGQANEGHACVKGRFAWGYATHKDRITKPMIRAKITDPWREVSWEEAINYAA
SEFRRIQAKHGRDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLK
TTLGESAGTQTFASVDKADVIMVIGANPTDGHPVFASRLKRRVREGAKLIVVDPRRIDIV
DTPHVKASHHLQLRPGTNVAVVNALAHVIVTEGLLNEAFIAERCEGRAFEQWRDFVALPE
NAPEATEAVTGVPAKDVREAARIYATGGNAAIYYGLGVTEHAQGSTMVMGIANLAMATGN
IGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDTIVRSQFEDAWNVSLQTEPGLR
IPNMFEAALDGSFMGLYCQGEDIVQSDPNTHHVAAALSAMECIVVQDIFLNETAKYAHVL
LPGSTFLEKDGTFTNAERRISRVRKVMPPVPGYADWEVTLLLSRALGYEMDYTHPSEIMD
EIARLTPTFHGVSYKKIDELGSIQWPCNEEAPDGTPTMHVDTFVRGKGKFVITKFIASPE
KVTRKFPLLLTTGRILSQYNVGAQTRRTENSRWHDEDRLELHPHDAEERGIKTGDWVGIE
SRAGQTVLRAKVTERMQPGVVYTTFHFPESGANVITTESSDWATNCPEYKVTAVQVMPVE
QPSEWQKEYSRFNAEQLELLKQRELANATSGK