Protein Info for ABIE53_003163 in Paraburkholderia graminis OAS925

Annotation: 2-polyprenyl-6-hydroxyphenyl methylase/3-demethylubiquinone-9 3-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR01983: 3-demethylubiquinone-9 3-O-methyltransferase" amino acids 8 to 218 (211 residues), 270.7 bits, see alignment E=4e-85 PF06325: PrmA" amino acids 36 to 150 (115 residues), 36.2 bits, see alignment E=2.4e-12 PF01209: Ubie_methyltran" amino acids 40 to 152 (113 residues), 25.5 bits, see alignment E=4.1e-09 PF13489: Methyltransf_23" amino acids 46 to 198 (153 residues), 85.6 bits, see alignment E=1.6e-27 PF13847: Methyltransf_31" amino acids 47 to 152 (106 residues), 52.4 bits, see alignment E=2.5e-17 PF05175: MTS" amino acids 47 to 119 (73 residues), 23.5 bits, see alignment E=1.9e-08 PF02353: CMAS" amino acids 47 to 176 (130 residues), 33.5 bits, see alignment E=1.4e-11 PF07021: MetW" amino acids 47 to 135 (89 residues), 21.1 bits, see alignment E=1.1e-07 PF13649: Methyltransf_25" amino acids 51 to 144 (94 residues), 63.1 bits, see alignment E=1.6e-20 PF08242: Methyltransf_12" amino acids 52 to 146 (95 residues), 57.6 bits, see alignment E=8.6e-19 PF08241: Methyltransf_11" amino acids 52 to 148 (97 residues), 69.1 bits, see alignment E=2.2e-22

Best Hits

Swiss-Prot: 94% identical to UBIG_PARPJ: Ubiquinone biosynthesis O-methyltransferase (ubiG) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00568, 3-demethylubiquinone-9 3-methyltransferase [EC: 2.1.1.- 2.1.1.64] (inferred from 98% identity to bgf:BC1003_2610)

MetaCyc: 56% identical to bifunctional 3-demethylubiquinol 3-O-methyltransferase/polyprenyldihydroxybenzoate methyltransferase (Xanthomonas campestris pv. campestris)
3-demethylubiquinone-9 3-O-methyltransferase. [EC: 2.1.1.64]; Hexaprenyldihydroxybenzoate methyltransferase. [EC: 2.1.1.64, 2.1.1.114]

Predicted SEED Role

"3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64)" in subsystem Ubiquinone Biosynthesis (EC 2.1.1.64)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.114 or 2.1.1.64

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (242 amino acids)

>ABIE53_003163 2-polyprenyl-6-hydroxyphenyl methylase/3-demethylubiquinone-9 3-methyltransferase (Paraburkholderia graminis OAS925)
MTNADPHELQKFSDLAHRWWDPNAEFKPLHELNPVRLNWIDAHAHLAGKHVLDIGCGGGI
LSESMAGLGAHVKGIDLSSEALGVADLHSLESGVTVSYEEIAAEALAAREPGTYDVVTCM
EMLEHVPQPSAIVEACKTLVKPGGWVFFSTLNRNVKSYLFAVIGAEYIARMLPKGTHDYA
RFIRPSELAGFVRAAGLRTADIKGIVYHPAQQALRTVRRHERELHARLPPRRVIPHCRQE
NQ