Protein Info for ABIE53_003066 in Paraburkholderia graminis OAS925

Annotation: gluconate 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF00389: 2-Hacid_dh" amino acids 4 to 309 (306 residues), 83.5 bits, see alignment E=2.3e-27 PF02826: 2-Hacid_dh_C" amino acids 107 to 284 (178 residues), 206.8 bits, see alignment E=3.6e-65 PF03446: NAD_binding_2" amino acids 146 to 255 (110 residues), 24.5 bits, see alignment E=4.8e-09 PF03807: F420_oxidored" amino acids 146 to 209 (64 residues), 25.2 bits, see alignment E=4e-09

Best Hits

Swiss-Prot: 48% identical to GYAR_THEKO: Glyoxylate reductase (gyaR) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

KEGG orthology group: None (inferred from 96% identity to bgf:BC1003_2536)

MetaCyc: 48% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.43, 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.43 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>ABIE53_003066 gluconate 2-dehydrogenase (Paraburkholderia graminis OAS925)
MQKILVARPIFPDVIERLQQYFDVDWNQGDVLPAEELKRRLADKDGALTAGDPIGADVLA
AAPRLRVVSNMAVGYNNFDMAAFNAANVLGTNTPDVLNESTADFGWALMMAAARRIAESE
HWLRAGKWQKWSYDGFLGGDIHGSTLGVIGMGRIGQALARRARGFNMQVIYHNRSRVAPE
IEAELNAEYVSKQDLLRRADHVVLVLPYTQENHHTIGAAELALMKPTATLTNIARGGIVD
DAALVQALRAKQIAAAGLDVFEGEPNLNPDLLTVPNIVLTPHIASATEATRRAMANLAAD
NLIAGLGEGPRAGQPPNPINAEVIGRARA