Protein Info for ABIE53_002884 in Paraburkholderia graminis OAS925

Annotation: pimeloyl-ACP methyl ester carboxylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 transmembrane" amino acids 130 to 150 (21 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 43 to 158 (116 residues), 44.3 bits, see alignment E=2.7e-15 PF12697: Abhydrolase_6" amino acids 43 to 266 (224 residues), 53.2 bits, see alignment E=9.9e-18 PF12146: Hydrolase_4" amino acids 71 to 163 (93 residues), 33.1 bits, see alignment E=5.7e-12

Best Hits

KEGG orthology group: None (inferred from 87% identity to bug:BC1001_2396)

Predicted SEED Role

"benzoate degradation ring-cleavage hydrolase" in subsystem Benzoate transport and degradation cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (276 amino acids)

>ABIE53_002884 pimeloyl-ACP methyl ester carboxylesterase (Paraburkholderia graminis OAS925)
MQNPAVETPASQFADLPATASHGPLRIEYRWLNAAANAAPIAVFLHEGLGSIAMWRDWPQ
TLCERLGMRGLVYSRPGYGRSTPREHHVKWPMHFMTDQARDVLPALLDALHIDTRERRRM
WVIGHSDGGSIALLYAALFPNALAGAVAIAPHLFVEDVSVQSIAQTKVLYETTDLRDKLS
RYHADVDSAFYGWNDVWLNPAFRQWSIAEQLTSIDKPLLAVQGHDDNYGTMAQIDTIQDY
VPHAQLARLDACGHSPHRDAPDRLNDVIAAFVSEHQ