Protein Info for ABIE53_002735 in Paraburkholderia graminis OAS925

Annotation: membrane protease subunit HflK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 transmembrane" amino acids 88 to 109 (22 residues), see Phobius details PF12221: HflK_N" amino acids 18 to 71 (54 residues), 37.7 bits, see alignment 1.6e-13 TIGR01933: HflK protein" amino acids 106 to 379 (274 residues), 264.3 bits, see alignment E=5.9e-83 PF01145: Band_7" amino acids 108 to 293 (186 residues), 89.3 bits, see alignment E=3.2e-29

Best Hits

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 88% identity to bug:BC1001_2247)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (470 amino acids)

>ABIE53_002735 membrane protease subunit HflK (Paraburkholderia graminis OAS925)
MNDYNERSIWLRMRAMLSLNDPRWGRGDGNGERQRPVEPKRPQAKEGEGPPDLDEMWRDF
NRRLSRMFGRKGGGAGGGRPDNGRGARIGVGIVIGVLIAIYLGSGVFVVQDGQAGVVMQF
GKYRYTAAHGVHWRLPYPFETHELVNIGQVRQVEIGRNNVVRLANVKDASMLTHDADIVD
IRFAVQYQIRKPTDYLFRSVDPDQSVMQAAQAAVRGIVGARSTQDILGQDREAIRQQLIA
AIQKSLDQYQSGLAVTGVTIQAVQAPDQVQAAFDDAARVRQENERAKRDAQAYAAELLPR
AQADVARQIDDAKKYSDKTVAQAQGDADRFKEVYAQYSKAPAVIRQRMYLETMQQIYSNT
TKVFVDNKSGNNVLYLPLDKLVEQNRQRVADAAAAAAASGAAAAGASQPANSPGAVAAQA
NAASAAAASTAASGSASAAAATPASRAAASSDALRSRESFRSRMREDDVQ