Protein Info for ABIE53_002731 in Paraburkholderia graminis OAS925

Annotation: adenylosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00184: adenylosuccinate synthase
  • amino acids 15 to 446 (432 residues), 583.1 bits, E=1.6e-179
• PF00709: Adenylsucc_synt
  • amino acids 15 to 440 (426 residues), 597.6 bits, E=5.8e-184

Best Hits

Swiss-Prot: 99% identical to PURA_PARPJ: Adenylosuccinate synthetase (purA) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 98% identity to bug:BC1001_2243)

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Protein Sequence (448 amino acids)

>ABIE53_002731 adenylosuccinate synthase (Paraburkholderia graminis OAS925)
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
ILRLIPSGIMHAGVACYIGNGVVLSPEALFKEIGELEAAGVDVQNRLFISEATTLILPYH
IAIDQGREARRGASKIGTTGRGIGPAYEDKVARRGLRVQDLFEPETFAERLRENLDYHNF
VLTQYLGVAAVDFQQTLDTMLSYADRLKPMVTDVSRRLYDANAAGSNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAATAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNAAR
QEAIGLELATVGKEFGSVTGRPRRTGWLDVAALRRSIQINGVSGLCMTKLDVLDGLDEVK
LCVGYTVDGKNVDLLPRGSSEVARCVPVYETFAGWKESTVGIKEWDKLPANARAYLSRVQ
EVAGIPIDMVSTGPDRDETILLRHPFKA