Protein Info for ABIE53_002544 in Paraburkholderia graminis OAS925

Annotation: glutamyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00464: glutamate--tRNA ligase
  • amino acids 5 to 466 (462 residues), 543.8 bits, E=1.9e-167
• PF00749: tRNA-synt_1c
  • amino acids 5 to 311 (307 residues), 323.9 bits, E=9.1e-101
• PF19269: Anticodon_2
  • amino acids 325 to 468 (144 residues), 108.5 bits, E=3.8e-35

Best Hits

Swiss-Prot: 95% identical to SYE_PARPJ: Glutamate--tRNA ligase (gltX) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 98% identity to bug:BC1001_2203)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (27/33 steps found)
• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (27/36 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (469 amino acids)

>ABIE53_002544 glutamyl-tRNA synthetase (Paraburkholderia graminis OAS925)
MTTSVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSTTESVDAIL
EGMEWLGLDFDEGPFYQMQRMDRYREVLAQMQSAGLVYPCYMSTEELDALRERQREAGEK
PRYDGTWRPEPGKVLPEPPAGVQPVLRFRNPLTGVVAWDDAVKGRIEISNEELDDLVIAR
PDGTPTYNFCVVVDDLDMRITHVIRGDDHVNNTPRQINILRALGAEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
PAQYDHDKLNWLNAHYIKEADNARLAGLARPFLADLGIDEATIAQGADLTAVVGLLKDRA
STVKEIAQNAAMFYRTPAPDAEALAQHVTDAVRPALGELAAALKTVEWTKEAIAAALKAT
LSAHKLKMPQLAMPVRLLVAGTTHTPSIDTVLMLFGREVVVSRIEKALA