Protein Info for ABIE53_002529 in Paraburkholderia graminis OAS925

Annotation: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00097: hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
  • amino acids 8 to 265 (258 residues), 319.9 bits, E=5.2e-100
• PF08543: Phos_pyr_kin
  • amino acids 15 to 263 (249 residues), 306.2 bits, E=1.6e-95
• PF00294: PfkB
  • amino acids 124 to 239 (116 residues), 30.2 bits, E=3e-11

Best Hits

Swiss-Prot: 60% identical to THID_ECOLI: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD) from Escherichia coli (strain K12)

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 94% identity to bxe:Bxe_A1658)

MetaCyc: 60% identical to bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Escherichia coli K-12 substr. MG1655)
Phosphomethylpyrimidine kinase. [EC: 2.7.4.7]; Hydroxymethylpyrimidine kinase. [EC: 2.7.4.7, 2.7.1.49]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.49, 2.7.4.7

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Protein Sequence (269 amino acids)

>ABIE53_002529 hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (Paraburkholderia graminis OAS925)
MTQSIPNVLTIAGSDSGGGAGIQADLKAFSALGAYGASVITALTAQNTRGVTAIHAPDAG
FVGAQLDAVFDDIRIDAVKIGMLANASIVRAVADALRRHKPAHIVLDTVMISKSNHALLL
PDAVAALRDELLPLADLLTPNLPEAAALLGGAQAAADEAAMLEQGEALRALGARAVLMKG
GHLNAADSPDWLVQESGTLRLGGPRVPVKNTHGTGCTLSSAIAALIPQRGDLASAVADAK
EYLTGALQASDRLDVGSGVGPVHHFYRWW