Protein Info for ABIE53_002527 in Paraburkholderia graminis OAS925
Annotation: alanine racemase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to ALR_PARPJ: Alanine racemase (alr) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 98% identity to bgf:BC1003_1267)MetaCyc: 58% identical to alanine racemase 2 (Escherichia coli K-12 substr. MG1655)
Alanine racemase. [EC: 5.1.1.1, 5.1.1.10]
Predicted SEED Role
"Alanine racemase (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions (EC 5.1.1.1)
MetaCyc Pathways
- anaerobic energy metabolism (invertebrates, cytosol) (7/7 steps found)
- L-alanine degradation I (2/2 steps found)
- alanine racemization (1/1 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- vancomycin resistance II (1/3 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (1/6 steps found)
- L-lysine degradation V (3/9 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
- superpathway of L-lysine degradation (12/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cysteine metabolism
- D-Alanine metabolism
- D-Arginine and D-ornithine metabolism
- D-Glutamine and D-glutamate metabolism
- Glycine, serine and threonine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.1.1.1 or 5.1.1.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (356 amino acids)
>ABIE53_002527 alanine racemase (Paraburkholderia graminis OAS925) MPRPLSATIHTSALANNLAVARRYAPKSKIWAVVKANAYGHGLARAFPGLRATDGFGLLD LEEAVKLRELGWAGPILLLEGFFRPTDIDVIDRYSLTTALHSDEQLRMLEMARLSKPVNI QLKMNSGMNRLGYTPEKFRAAWERARACQGVGQITLMTHFSDADSERGVAHQMEAFERGA QGIAGARSLANSAATLWHPQSHFDWVRPGIILYGASPSGVSAAIQGTGLQPAMTLASELI AVQNIAEGQTVGYGSLFKARGPMRIGVVACGYADGYPRVAPEGTPVIVDGVRTRIVGRVS MDMLTVDLTPVPTANVGSRVELWGASLPIDDVARACGTIGYELMCAVAPRVPVRAE