Protein Info for ABIE53_002458 in Paraburkholderia graminis OAS925

Annotation: DHA2 family multidrug resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 87 to 110 (24 residues), see Phobius details amino acids 117 to 136 (20 residues), see Phobius details amino acids 146 to 167 (22 residues), see Phobius details amino acids 173 to 195 (23 residues), see Phobius details amino acids 207 to 226 (20 residues), see Phobius details amino acids 238 to 259 (22 residues), see Phobius details amino acids 279 to 301 (23 residues), see Phobius details amino acids 312 to 329 (18 residues), see Phobius details amino acids 341 to 358 (18 residues), see Phobius details amino acids 370 to 393 (24 residues), see Phobius details amino acids 409 to 422 (14 residues), see Phobius details amino acids 482 to 502 (21 residues), see Phobius details PF06609: TRI12" amino acids 16 to 272 (257 residues), 34.8 bits, see alignment E=1e-12 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 21 to 501 (481 residues), 511.4 bits, see alignment E=1.3e-157 PF07690: MFS_1" amino acids 26 to 418 (393 residues), 179.4 bits, see alignment E=1.4e-56 PF00083: Sugar_tr" amino acids 53 to 188 (136 residues), 24 bits, see alignment E=2.6e-09

Best Hits

Swiss-Prot: 53% identical to EMRB_ECO57: Multidrug export protein EmrB (emrB) from Escherichia coli O157:H7

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 97% identity to bgf:BC1003_1995)

MetaCyc: 53% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>ABIE53_002458 DHA2 family multidrug resistance protein (Paraburkholderia graminis OAS925)
MAQAQAQAPHPPLEGAQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSADQGTWV
ITSFAVANAISVPLTGWLTDRIGQVRLFMASIVLFVISSWMCGLAPTLPFLLASRVLQGA
VAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAGPILGGWISDNISWPWIFYVN
IPVGAVAAAATWMIFRNRDSVVKKAPIDGVGLGLLILWVGSLQVMLDKGKDLDWFSSTTI
VVLALVAVIALAFFIVWELTAEHPVVDLSLFSKRNFTGGTVALSIGYGLYFGNLVLLPLW
LQTDIGYTATDAGLVMAPVGLFAVLLSPITGKVLPRTDPRYIATLSFLVFALCFWMRSRY
TTGVDTYSLLLPTLIQGIGMAGFFIPLVSITLSGLPGNRIPAASGLSNFVRIMCGGIGTS
IFQTAWDHRSIMHHAQLTERANAYNPVFDQSIRQMGAAGFSQPQAYGLFNSMATQQAAQL
GVNDLFFVSAGIFVALIALIWITKPERAGGDAGAAAAAAH