Protein Info for ABIE53_002416 in Paraburkholderia graminis OAS925

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 129 to 236 (108 residues), 70.2 bits, E=3.8e-23
• PF00441: Acyl-CoA_dh_1
  • amino acids 363 to 504 (142 residues), 57.8 bits, E=3e-19
• PF09317: ACDH_C
  • amino acids 517 to 712 (196 residues), 296 bits, E=7.2e-92

Best Hits

KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 95% identity to bug:BC1001_1426)

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Brassinosteroid biosynthesis
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid metabolism
• Geraniol degradation
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-, 1.3.99.2

Use Curated BLAST to search for 1.3.99.- or 1.3.99.2

Protein Sequence (842 amino acids)

>ABIE53_002416 acyl-CoA dehydrogenase (Paraburkholderia graminis OAS925)
MSWFVLLLVVGALALVYVQARALWWLVFMVAWVAAVRVSGAAGVAATTLFAIVLVLPALV
LAVKPLRRAWLAKPVLDTFRKILPDMSPTERDAIEAGTVWWDAELFSGRPHWDTLLGYGP
ATLTAEEQSFLDNECEQLCDLANDWETTMVWQDLSPQAWQFIKERGFLGMIIPKQYGGKQ
FSAYAHSQVIMKLATRCSAAAVSVMVPNSLGPAELLMHYGTDEQKNHYLPRLARGDEIPC
FALTSPYAGSDAAAIPDVGIVCKGMHEGRETLGFRVTWDKRYITLGPIATVLGLAFRALD
PDRLLGSNDEPGITCALIPTDHPGVNIGRRHWPLNAVFQNGPNSGKDVFIPLEWVIGGRA
QVGNGWRMLMECLAAGRAISLPSSNVGMAKLAVRGTGAYAAVRRQFRTAVGKFEGVQEAL
GRMGGNLYVMDAARRLSAHAVDLGEKPSVISAIAKYHITERARMVINDAMDVTAGKGICM
GPSNFLARAYQQVPIAITVEGANILTRCLIIFGQGAIRCHPYVLKEMSATREADRAKALR
DFDDAFFGHVGFTLSNVVRSVVYGITGGAFIATPRAAHAPLHAYYRAATRIATVFALLAD
VSMFVLGGDLKRRERISARLGDVLSQLYLISATLKRFEDEGRQEDDLPLVRWGVEDSLYR
AQQALDGVLANYPNRLAAGLVRVLAFPFGLPHREPSDRLGSAIAELMQTPGAGAQPAGVR
LVRAASRRRCAGLRRTRVRAEPALHADRAEASRSGESAPPRADAAERRATGRMGRRRAAA
RPDRRRRAPRARRVRALRGTRREGRRLPRRLRPARQSATTQGRTRNGARSRAVTRSRARA
SP