Protein Info for ABIE53_002407 in Paraburkholderia graminis OAS925

Annotation: protein-L-isoaspartate(D-aspartate) O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 12 to 219 (208 residues), 206.1 bits, see alignment E=2.9e-65 PF01135: PCMT" amino acids 15 to 218 (204 residues), 206.3 bits, see alignment E=7.5e-65 PF13649: Methyltransf_25" amino acids 89 to 158 (70 residues), 27 bits, see alignment E=8.9e-10 PF05139: Erythro_esteras" amino acids 336 to 686 (351 residues), 438.7 bits, see alignment E=3.8e-135

Best Hits

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (708 amino acids)

>ABIE53_002407 protein-L-isoaspartate(D-aspartate) O-methyltransferase (Paraburkholderia graminis OAS925)
MDESTVNEEAFDEARQQMVESQIAARGVRDARVLDAMREVPRHLFVPPELAEFAYSDTPL
PLDDGQTISQPVIVARMLEAAQLSPGDRVLDVGTGSGYAAAVASRIVAHVDSIERHASLA
DSARQALHEHAFDNVVVHHADGTRGWPAAAPFDAIIAAAGGPDVPQAWREQLAIGGRLVM
PVGASTETQRLIKVTRRSDTEFDEEDICGVHFVPLIGEQGWPEEDGVAAAGAEQSSEAGG
GVSVDEPQGQERGPTRGRTQRPTQRPTPKQARTQRQPHSLAGLIAASARPLPEPEDETFA
DAFDHLRTKRVVLLGECSHGTSEFYRARAAITRRLVERHGFMIVAVEADWPDAAVIDSYA
RAREPRNGEPPFQRFPVWMWRNEEFAAFVRWLRAHNEQQSDGRRCGFYGLDMYSLSASIA
AVLDYLDRTDPEAARIARERYGCLTPWQKDPQVYGRAAFSAGFRTCENAVIQQLQDLLRK
RLDEANVDGEHWFDATQNARLVASAERYYRTMYRSSAASWNLRDTHMFETLESLLDSQGP
DSRAVVWAHNSHIGNAAATEMGRVRNELNVGQLCRERFGDAAALIGFGTHAGDVAAASDW
DGPMEIKAVRPSREDSYEYQFHASGEPRCVVDLSNGADALLRARLSEARLERFIGVIYRP
ETELHSHYAEAVLPEQFDAYVWFDTTRAVEPLATRATERAPDLYPFGV