Protein Info for ABIE53_002336 in Paraburkholderia graminis OAS925

Annotation: gluconate 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF00389: 2-Hacid_dh" amino acids 7 to 315 (309 residues), 95.7 bits, see alignment E=2.9e-31 PF02826: 2-Hacid_dh_C" amino acids 104 to 283 (180 residues), 223.2 bits, see alignment E=2.5e-70 PF03446: NAD_binding_2" amino acids 144 to 251 (108 residues), 24 bits, see alignment E=5.3e-09

Best Hits

Swiss-Prot: 58% identical to GHRB_SERP5: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 94% identity to bgf:BC1003_1871)

MetaCyc: 55% identical to glyoxylate reductase (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; Gluconate 2-dehydrogenase. [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215, 1.1.1.79

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43 or 1.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>ABIE53_002336 gluconate 2-dehydrogenase (Paraburkholderia graminis OAS925)
MKKIVAWKSLPEDVLAYLQQHVEVVQVDPARHDAFVAALREADGGIGASVKITPAMLEGA
TRLKALSTISVGFDQFDVADLTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAAW
VKAGQWQHSIGPALFGVDVQGKTLGIVGLGRIGGAVARRAALGFNMQVLYTNRSANPEAE
AAYGARRVELAELLATADFVCLQVPLTEETRHMIGAKELASMKKSAILINASRGAIVDES
ALIDALRNGTIHGAGLDVFEKEPLPADSPLLQMANVVALPHIGSATHETRHAMARNAAEN
LVAALAGTLTTNIVNREVLQR