Protein Info for ABIE53_002314 in Paraburkholderia graminis OAS925

Annotation: MHS family alpha-ketoglutarate permease-like MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 60 to 81 (22 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 158 to 181 (24 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 280 to 299 (20 residues), see Phobius details amino acids 310 to 328 (19 residues), see Phobius details amino acids 334 to 358 (25 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 403 to 421 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 23 to 219 (197 residues), 85.5 bits, see alignment E=7.6e-28 amino acids 224 to 417 (194 residues), 41.2 bits, see alignment E=2.1e-14 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 28 to 415 (388 residues), 461.7 bits, see alignment E=1.1e-142 PF07690: MFS_1" amino acids 66 to 275 (210 residues), 57.7 bits, see alignment E=2e-19 amino acids 266 to 428 (163 residues), 55.9 bits, see alignment E=7.1e-19

Best Hits

Swiss-Prot: 74% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 96% identity to bug:BC1001_1528)

MetaCyc: 74% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>ABIE53_002314 MHS family alpha-ketoglutarate permease-like MFS transporter (Paraburkholderia graminis OAS925)
MTDLTDHSVASAHDTRRRIFAIVGASSGNLVEWFDFYVYSFCALYFAPAFFPSGNTTTQL
LNTAGVFAAGFLMRPIGGWFFGRLADKHGRRTAMMISVFMMCGGSLVIAVLPTYAQIGAL
APALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLCALLVLVI
LQQTLSTAELKAWGWRVPFVIGAIAALVALYLRKSLDETTTAATRQRKEAGTLRGLWQHR
VAFMTVLGFTAGGSLIFYTFTTYMQKYLVNTAGMNAKTASNVMTAALFVYMLLQPLFGAL
SDRIGRRRSMLCFGLFATIGTVPLLHALKDVTSPYAAFGLVVLALAIVSFYTSISGLIKA
EMFPPEVRALGVGLSYAVANAIFGGSAEYVALWLKSTGSEPMFYWYVTLMCAIAGIVALR
MRDPSKEGYLRHEP