Protein Info for ABIE53_001877 in Paraburkholderia graminis OAS925

Annotation: DHA2 family multidrug resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details transmembrane" amino acids 57 to 77 (21 residues), see Phobius details amino acids 86 to 109 (24 residues), see Phobius details amino acids 115 to 134 (20 residues), see Phobius details amino acids 145 to 166 (22 residues), see Phobius details amino acids 172 to 194 (23 residues), see Phobius details amino acids 206 to 224 (19 residues), see Phobius details amino acids 236 to 256 (21 residues), see Phobius details amino acids 277 to 299 (23 residues), see Phobius details amino acids 311 to 329 (19 residues), see Phobius details amino acids 340 to 358 (19 residues), see Phobius details amino acids 372 to 394 (23 residues), see Phobius details amino acids 407 to 423 (17 residues), see Phobius details amino acids 482 to 502 (21 residues), see Phobius details PF06609: TRI12" amino acids 19 to 301 (283 residues), 35 bits, see alignment E=6.1e-13 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 19 to 502 (484 residues), 475.6 bits, see alignment E=9e-147 PF07690: MFS_1" amino acids 24 to 418 (395 residues), 180.5 bits, see alignment E=4.6e-57

Best Hits

Swiss-Prot: 53% identical to EMRB_ECOLI: Multidrug export protein EmrB (emrB) from Escherichia coli (strain K12)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 89% identity to bpy:Bphyt_2415)

MetaCyc: 53% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>ABIE53_001877 DHA2 family multidrug resistance protein (Paraburkholderia graminis OAS925)
MNSSTQLAPPPLSGAKLVLATFAVALATFMNVLDSSIANVAIPTISGNLGVSVDEGTWVI
TLFSAANAVAIPLTGWLTQRVGQIKLFVAAIVLFVFSSWLCGVAPNLVILLAARVLQGAV
AGPLIPLSQAILLGSYPKEKSSTALALWAMTATVGPIAGPALGGWITDSYSWSWIFYINI
PVGLFAAAVTWFIYRDRETPARKLPIDKVGLMSLVAWVASLQIMLDKGKDLDWFSSPVIC
ILAAVAAVSFVFFLIWEFTEKNPIVDLRLFAVRNFRGGTIAISVAYAVFFANLVILPQWI
QGYLGYRSVDAGLVTAPLGIFAVLLAPVMAKIMPKTDARVLATLAFVGFAAVFLMRSHYT
TGVDTYTLVLPTLLQGIPMALFFTPLTAIILSGLSPDKIPAAAGLSNFVRVFAGGVGTSL
ITTGWNNRTILHHAQLAEQSSVNNPAYTSALADIHTTLGGNMDQAAAFFERSLNAQAAML
GLNDIFWLSSMIFIVIIPLIWLTKPGKGGGGGAAAGAH