Protein Info for ABIE53_001876 in Paraburkholderia graminis OAS925

Annotation: membrane fusion protein (multidrug efflux system)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 49 to 70 (22 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 89 to 133 (45 residues), 25 bits, see alignment 3.3e-09 PF16576: HlyD_D23" amino acids 89 to 316 (228 residues), 42.9 bits, see alignment E=8.8e-15 PF00529: CusB_dom_1" amino acids 177 to 373 (197 residues), 35.3 bits, see alignment E=2.3e-12 PF13437: HlyD_3" amino acids 245 to 324 (80 residues), 54 bits, see alignment E=6.3e-18

Best Hits

Swiss-Prot: 51% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 85% identity to bph:Bphy_4021)

MetaCyc: 51% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>ABIE53_001876 membrane fusion protein (multidrug efflux system) (Paraburkholderia graminis OAS925)
MSEINTPERAATHAAPANVDTATAPNAAAATAATAPTAASEPNSRKRQLLLTLLGAAVVV
SVAAYGAYYMTYARYHETTDDAYVSGNLVQLTPQVTGTVVAVNADDTQIVKAGDPVVTLD
NADATVALGNAEAMLGQTVRQVSSLYVNNDFYAANIAQKQSDLARAQDDLRRRQAVADTG
AVSAEDIAHARDTVTAAQAALDAARQQAEANRALTDHTTIEQHPSVQAAASKVRDAYLAY
ARNTLPAPVTGYVAKRSVQVGQRVSPGTPLMAVVPLNGVWVDANYKESQLRNMRIGQPVT
LTADVYGSKVEYHGRVVGFSAGTGSAFATLPAQNATGNWIKVVQRLPARIELDQKELQAH
PLRIGLSMQVDVDTRDSTGAQLGAAVNTTYRTDVFAQYGAQADAEIQKIIERNSVVAHPG
PTERVVSSAQGTRDKRNSAAMPKRAG