Protein Info for ABIE53_001742 in Paraburkholderia graminis OAS925

Annotation: starch synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 PF08323: Glyco_transf_5" amino acids 7 to 174 (168 residues), 197.4 bits, see alignment E=8.2e-62 TIGR02095: glycogen/starch synthase, ADP-glucose type" amino acids 10 to 415 (406 residues), 510.3 bits, see alignment E=2.5e-157 PF13439: Glyco_transf_4" amino acids 35 to 162 (128 residues), 30.7 bits, see alignment E=7.5e-11 PF00534: Glycos_transf_1" amino acids 222 to 392 (171 residues), 59.3 bits, see alignment E=9.1e-20 PF20706: GT4-conflict" amino acids 227 to 406 (180 residues), 26.7 bits, see alignment E=6.7e-10 PF13692: Glyco_trans_1_4" amino acids 234 to 377 (144 residues), 48.7 bits, see alignment E=2.5e-16

Best Hits

Swiss-Prot: 59% identical to GLGA_FRATN: Glycogen synthase (glgA) from Francisella tularensis subsp. novicida (strain U112)

KEGG orthology group: K00703, starch synthase [EC: 2.4.1.21] (inferred from 95% identity to bgf:BC1003_2174)

MetaCyc: 56% identical to glycogen synthase (Escherichia coli K-12 substr. MG1655)
Starch synthase. [EC: 2.4.1.21]

Predicted SEED Role

"Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)" in subsystem Glycogen metabolism (EC 2.4.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.21

Use Curated BLAST to search for 2.4.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>ABIE53_001742 starch synthase (Paraburkholderia graminis OAS925)
MTLEQGTLPSNGLVVYMIRAATLYDRPGNPYLNDEHVPYGDNAQRFALLGWVAAQLAQQL
DPAWSPQIVHAHDWHAGLAAAYLKAAERQHGRPPARSVFTVHNLAYQGVFPAHQFGQLGL
PDDFFNMRGVEFYGQLSFLKAGLYYSDRITTVSPTYAREIQTLAQGGGLDGLLRSRAHDL
CGILNGVDYTVWNPANDALLEHRYTATRLAGKLACKEALQKRFGLAQKSDALLFGVVSRL
TEQKGLDLLLETVPEIVKRGGQLVVFGTGDPALENGLKRVAQTHPESVAVELGFDEKLAH
TIIAGSDVIAVPSRFEPCGLTQLYALAYGSLPLVHRVGGLADTVADASLENLAEDLATGF
VFDDFTPRSLTAAIRRAFALHGRRTEWKAAQRRAMRQDFGWGASADRYLALYRELA