Protein Info for ABIE53_001158 in Paraburkholderia graminis OAS925

Annotation: UDP-glucose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 15 to 218 (204 residues), 34.6 bits, E=3.9e-12
• PF01370: Epimerase
  • amino acids 17 to 251 (235 residues), 169.8 bits, E=2.4e-53
• PF05368: NmrA
  • amino acids 17 to 132 (116 residues), 25.5 bits, E=2.9e-09
• PF02719: Polysacc_synt_2
  • amino acids 17 to 270 (254 residues), 31.6 bits, E=3.5e-11
• PF16363: GDP_Man_Dehyd
  • amino acids 17 to 313 (297 residues), 138.6 bits, E=1.2e-43
• PF01073: 3Beta_HSD
  • amino acids 17 to 238 (222 residues), 59.4 bits, E=1.1e-19
• PF13460: NAD_binding_10
  • amino acids 20 to 184 (165 residues), 54.7 bits, E=4.3e-18

Best Hits

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 52% identity to pmt:PMT0086)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (335 amino acids)

>ABIE53_001158 UDP-glucose 4-epimerase (Paraburkholderia graminis OAS925)
MYSHASREIAASMKRCLVLGGGGFIGSHLTRALLEHGYYVRCFERPHTGARCPQAIEHPN
FELFQGDFVNPLDLACALEGVDVCFHLVSTTLPKSSNADPAFDVETNLLGTVRLLTQAVK
SGVTKVIFVSSGGTVYGVPQAVPVPETHPTNPVCSYGIAKLAIEKYLALFHQQHGLDYAV
LRLANPFGEGQRAESGQGAVAVFLGRILRGETLEVWGDGSVIRDYIYIEDVVTALLLSID
LKGSERLFNIGSGRGHSINEVLDVLERTVGQPAIRRYRSGRSFDVPTSVLCIESARRSLG
WSPKVDFEEGVERFAAWMKRELAAQSLQAIGGYSG