Protein Info for ABIE53_001140 in Paraburkholderia graminis OAS925

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 8 to 295 (288 residues), 334.4 bits, see alignment E=2.5e-104 PF04321: RmlD_sub_bind" amino acids 8 to 295 (288 residues), 358.5 bits, see alignment E=5.8e-111 PF01370: Epimerase" amino acids 9 to 164 (156 residues), 71.4 bits, see alignment E=2e-23 PF16363: GDP_Man_Dehyd" amino acids 40 to 144 (105 residues), 41.6 bits, see alignment E=2.9e-14 PF07993: NAD_binding_4" amino acids 47 to 159 (113 residues), 21.8 bits, see alignment E=2.3e-08

Best Hits

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 80% identity to bph:Bphy_2316)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>ABIE53_001140 dTDP-4-dehydrorhamnose reductase (Paraburkholderia graminis OAS925)
MSAGDQPTILLTGVNGQVGFELARTLQGLGKVVALERSALNLADLEQVRRVVREVKPALI
VNPAAHTAVDKAETDIDAVMRLNAEAPGVLAEEAKRLGAALVHYSTDYVFDGTKAGAYVE
TDAVNPQNVYGKSKLAGEQAIAQSGCAHLIFRTSWVYGARGKNFLLTMLRLGAERDELSV
VADQYGAPTWSNTIAALSANVLAQAVAPNQSDWWEKHSGVYHLTASGSTSWHGFAEAIFE
YSQLEKKPTVKPIPAASYPTPATRPSNSRMSNDKLAASFGVRAPDWRDALRLCMSTK