Protein Info for ABIE53_001028 in Paraburkholderia graminis OAS925

Annotation: hopanoid biosynthesis associated radical SAM protein HpnJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 TIGR03471: hopanoid biosynthesis associated radical SAM protein HpnJ" amino acids 1 to 472 (472 residues), 843 bits, see alignment E=3.4e-258 PF04055: Radical_SAM" amino acids 201 to 361 (161 residues), 73.9 bits, see alignment E=9.2e-25

Best Hits

KEGG orthology group: None (inferred from 96% identity to bgf:BC1003_1748)

Predicted SEED Role

"Fe-S oxidoreductase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (473 amino acids)

>ABIE53_001028 hopanoid biosynthesis associated radical SAM protein HpnJ (Paraburkholderia graminis OAS925)
MKTLFLQAPSYDGFDGGAGSRYQAKREVRSFWYPTWLAQPAALVPDSRVLDAPADGLSVD
ATLDIAQHYDLVVIHTSTPSFPTDALFAEDLKKRKPSLVIGMVGAKVAVDPHNSLTASEA
IDFVCREEFDFTCQEVAAGKPFAQILGLSYRAADGSIEHNAARPILENMDELPFVAPVYK
RDLKIDNYFIGYLKHPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRTRSVENVLAEVKWIR
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGKLGVTWSCNAKANVPYSTLKIMKENGLRL
LLVGYESGDDQILLNIRKGLRTDIARRFSDDCRKLGIKIHGTFILGLPGETQQTIQKTID
YAKEINPHTIQVSLAAPYPGTRLYDQAVENGWLEENKVINLVSKEGVQLAAIGYPHLSRE
EIYHQLENFYKRFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA