Protein Info for ABIE53_000974 in Paraburkholderia graminis OAS925

Annotation: dihydroxyacetone kinase-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 TIGR02363: dihydroxyacetone kinase, DhaK subunit" amino acids 1 to 324 (324 residues), 455.5 bits, see alignment E=4.9e-141 PF02733: Dak1" amino acids 18 to 323 (306 residues), 408.5 bits, see alignment E=7.9e-127

Best Hits

Swiss-Prot: 48% identical to DHAK1_LISIN: PTS-dependent dihydroxyacetone kinase 1, dihydroxyacetone-binding subunit DhaK (dhaK-1) from Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)

KEGG orthology group: K05878, dihydroxyacetone kinase, N-terminal domain [EC: 2.7.1.-] (inferred from 90% identity to bpy:Bphyt_5631)

Predicted SEED Role

"Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), dihydroxyacetone binding subunit DhaK" in subsystem Dihydroxyacetone kinases (EC 2.7.1.121)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.1.121

Use Curated BLAST to search for 2.7.1.- or 2.7.1.121

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>ABIE53_000974 dihydroxyacetone kinase-like protein (Paraburkholderia graminis OAS925)
MKKFINHVDDFLAESLAGFASAHSDLVVLNNEPVYVRRKTLKPGKVALISGGGSGHEPLH
SGFVGYGMLDAACPGQVFTSPTPDQMMAAADAVDTGAGVLFIVKNYSGDLMNFEMASEMS
EVPNAMVLINDDVAVENSSYTTGRRGVAGAVIVEKLVGSMAESGADLEQCKAFGDRINKR
TASMGVAFSSCTVPAAGTLTFKIGDDEIEVGVGIHGEPGRRRAKFAGADAIAGELLKAIV
DDLAPQPGAELLVLINGLGGTPLGELYLLFNSTRAWLEQRDLKVGRAQVGALTTSLEMAG
ASITLCMLDDEMTRHWDSPVHTPSLRWGM