Protein Info for ABIE53_000908 in Paraburkholderia graminis OAS925

Annotation: D-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 5 to 328 (324 residues), 112 bits, E=1.7e-36
• PF02826: 2-Hacid_dh_C
  • amino acids 110 to 298 (189 residues), 184.8 bits, E=9.8e-59

Best Hits

Swiss-Prot: 51% identical to LDHD_ECOLI: D-lactate dehydrogenase (ldhA) from Escherichia coli (strain K12)

KEGG orthology group: K03778, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 95% identity to bgf:BC1003_0507)

MetaCyc: 51% identical to D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
D-lactate dehydrogenase. [EC: 1.1.1.28]

Predicted SEED Role

"D-lactate dehydrogenase (EC 1.1.1.28)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.28)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• heterolactic fermentation (15/18 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• mixed acid fermentation (12/16 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• vancomycin resistance I (2/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.28

Use Curated BLAST to search for 1.1.1.28

Protein Sequence (340 amino acids)

>ABIE53_000908 D-lactate dehydrogenase (Paraburkholderia graminis OAS925)
MRMILFSSRQYDVETFTEANAAHRYELHFQESHLNSETAVLARAYEVVCPFVNDRVDAAT
LEHLHAGGTRMIALRSAGFNHVDLATAERLGIAVARVPAYSPHAVAEHAVGLVLALNRRL
PRAVARTREGDFSLHGLLGFDLHGKTVGVIGTGMIGRVFGRIMSGFGMRVLAYDPGTPAD
DLLALGARYVTLDALLAESDIVSLHCPLLPATYHLIDAAALARMKRGAMLINTGRGGLVE
SNALVGALKDGQLGHLGLDVYEEEGGLFFEDHSNLPLQDDVLARLLMFPNVIVTAHQAFF
TREAMNEIAQTTLANVAAWQAGTPRNAVHANDQASSGLPG