Protein Info for ABIE53_000870 in Paraburkholderia graminis OAS925

Annotation: poly(A) polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 533 TIGR01942: poly(A) polymerase" amino acids 70 to 510 (441 residues), 504.3 bits, see alignment E=1.3e-155 PF01743: PolyA_pol" amino acids 101 to 245 (145 residues), 125.7 bits, see alignment E=2.1e-40 PF12627: PolyA_pol_RNAbd" amino acids 272 to 332 (61 residues), 72.5 bits, see alignment E=2.9e-24 PF12626: PolyA_pol_arg_C" amino acids 386 to 499 (114 residues), 145.6 bits, see alignment E=1e-46

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 97% identity to bug:BC1001_0458)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (533 amino acids)

>ABIE53_000870 poly(A) polymerase (Paraburkholderia graminis OAS925)
MIKKLIRKLFGQDTASADDTLPAEADADETGSAPAHHAQGAAGAGRARRKPARGGASTKP
QVPDPDVPVIIPHDVHGIDPTLISRNAIRVTEGLQQAGHRAFIVGGAVRDLLLGIKPKDF
DVATDATPEQVQKLFRRARIIGRRFQIVHVQFGQEIIETSTFRALVDPPAADAAPPRRLK
RDELDRRTHAVDASGRVLRDNVWGEQHEDATRRDFTINAMYYDPATETVLDYHNGMADVR
ARLLRMIGDPATRYREDPVRMLRVVRFAAKLDFEIEDATRDPIAKMADLINNVPAARLFD
EMLKLMLSGHALACLKRLRQEGLHHGLLPLLDVVLEQPTGEKFITLALNNTDARVRAGKP
VSPGFLFATLLWHDVQQRWQKFEANGEYPVPALHRAMDEVLDMQTEKLAIHKRFSSDMRE
IWGLQLRLEKRSGRSALKLLEHQRFRAGYDFLLLRCESGELDESVGAWWTEFIEGDIAAR
EALLTQGGKDRSPRKRRRRGGGARNRKPGDGMEGGTPAERTDAEAGHDSPHED