Protein Info for ABIE53_000660 in Paraburkholderia graminis OAS925

Annotation: phosphotransferase system enzyme I (PtsI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 5 to 575 (571 residues), 539 bits, see alignment E=6.2e-166 PF05524: PEP-utilisers_N" amino acids 6 to 129 (124 residues), 107.5 bits, see alignment E=7.5e-35 PF00391: PEP-utilizers" amino acids 161 to 231 (71 residues), 82 bits, see alignment E=2.9e-27 PF02896: PEP-utilizers_C" amino acids 258 to 551 (294 residues), 374.1 bits, see alignment E=6.9e-116

Best Hits

Swiss-Prot: 69% identical to PT1_CUPNH: Phosphoenolpyruvate-protein phosphotransferase (phbI) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 99% identity to bgf:BC1003_0265)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (582 amino acids)

>ABIE53_000660 phosphotransferase system enzyme I (PtsI) (Paraburkholderia graminis OAS925)
MSFTLHGIPVSRGIAIGRAYLIAPAALDVDHYLIEPAQIESEVERFRTAQQRVHVELDAL
RADLAADAPSEMGAFINVHSMILNDAMLVQETIDLIRTRRYNVEWALTEQLERLSRHFDD
IEDEYLRERKADIEQVVERVLKALAGASATLVDGVHGACSEMIVVAHDIAPADMMQFKTQ
TFQGFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDHGIVIVDPAPI
VLEEYSYRQSEKALEQRKLQRLKFSPTQTLCGTRIELCANIELPDDARAALDAGATGVGL
FRTEFLFMNHKDRMPEEEEQFEAYRRAVELMNGLPVTIRTIDVGADKPLDSMGGGDGYET
APNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGTVKILVPMLAHAQEIDQTLDLIREA
KRQLDDAGIAYNPNVQVGAMIEIPAAAIALPLFLKRLDFLSIGTNDLIQYTLAIDRADNS
VAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPTLTRLLLGMGLTEFSMHPSQ
LLEVKQEVLRSHLKTLEKPVADVLASFEPEEVQAALKRVAQA