Protein Info for ABIE53_000425 in Paraburkholderia graminis OAS925

Annotation: 23S rRNA (cytosine1962-C5)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 PF17785: PUA_3" amino acids 4 to 67 (64 residues), 79.5 bits, see alignment E=5.6e-26 PF02475: Met_10" amino acids 137 to 274 (138 residues), 29.6 bits, see alignment E=2.7e-10 PF10672: Methyltrans_SAM" amino acids 173 to 362 (190 residues), 99.9 bits, see alignment E=7.1e-32 PF05175: MTS" amino acids 216 to 340 (125 residues), 28 bits, see alignment E=7.4e-10 PF06325: PrmA" amino acids 219 to 302 (84 residues), 23.1 bits, see alignment E=2.1e-08 PF03602: Cons_hypoth95" amino acids 220 to 306 (87 residues), 39.3 bits, see alignment E=2.5e-13 PF13847: Methyltransf_31" amino acids 227 to 361 (135 residues), 32.1 bits, see alignment E=4.3e-11 PF09445: Methyltransf_15" amino acids 228 to 308 (81 residues), 24 bits, see alignment E=1.2e-08 PF13649: Methyltransf_25" amino acids 228 to 303 (76 residues), 27.5 bits, see alignment E=1.9e-09

Best Hits

Swiss-Prot: 50% identical to RLMI_SHESW: Ribosomal RNA large subunit methyltransferase I (rlmI) from Shewanella sp. (strain W3-18-1)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 96% identity to bgf:BC1003_0110)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (400 amino acids)

>ABIE53_000425 23S rRNA (cytosine1962-C5)-methyltransferase (Paraburkholderia graminis OAS925)
MNIVTLKPSKEKSLLRRHPWVYANAIDRIDGNPAPGATVLVRAHDGRFLARAAYSPHSQI
RARVWSFDESEPIDHAFFKRRVQRALLHRQTMVRDTGAVRLIFGEADGLPGLIVDHYVAE
DEGQRSQLVCQFMAAGVEAWKEAIVAALVGATGCPNVYERSDVSIRQKEGLEQTTGVLAG
EAPSEALIASENGVRYHVDVRNGHKTGFYVDQRDNRLLVQQLAQDRDVLNCFCYTGGFSL
AALKGGAKRVVSIDSSGEALALAQQNVAANGFDAERATWLDADAFKTLRRLYDEGERFDL
IVLDPPKFAPSREHVDRASRAYKDINLTGLKLLRPGGLLFTYSCSGAIDSELFQKIVASA
AADARVDARILKRLGAGVDHPLLTAFPEGEYLKGLLLQIA