Protein Info for ABIE53_000394 in Paraburkholderia graminis OAS925

Annotation: nitrogen fixation/metabolism regulation signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 transmembrane" amino acids 14 to 38 (25 residues), see Phobius details amino acids 50 to 75 (26 residues), see Phobius details amino acids 87 to 109 (23 residues), see Phobius details amino acids 310 to 334 (25 residues), see Phobius details PF00672: HAMP" amino acids 333 to 383 (51 residues), 43.2 bits, see alignment 1e-14 PF00512: HisKA" amino acids 540 to 606 (67 residues), 37.6 bits, see alignment E=4.6e-13 PF02518: HATPase_c" amino acids 649 to 759 (111 residues), 67.4 bits, see alignment E=3.6e-22

Best Hits

KEGG orthology group: None (inferred from 97% identity to bug:BC1001_0056)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>ABIE53_000394 nitrogen fixation/metabolism regulation signal transduction histidine kinase (Paraburkholderia graminis OAS925)
MLNKVRSATSVSSIVVRVLVSTVAVTAVLLLVLLAAASANTEFFDRYYQWLYAANLVVAM
IFLLVVATLVIIIIVRLRKGKFGTRLLAKLAFFMALVGVVPGGIIYVVSYQFVSRSIESW
FDVNVETALTSGLNLGRGMLDASLSDLQTKGRLMSEQLASADAAGTTLTLLRLRDQFGVQ
EATIVEPTRSMSGATPDMHVVAQASSNYASLVPNDLPTPLMVDAARGRGYAAIEGEVDGD
PRAHGSKGALRLRVVQRIPDSNASLLQPTERFLQLTQPVSQSLARNADAVQRAYREYQEK
ALGRTGLRKMYIGTLTLALFLATFIAMMLALALGNQLARPLFLLAQGTKEITEGDYTPKR
EIKSRDELGFLTQSFNAMTRQLSEARAAVENNRIALEHSKAYLESILANLTAGVFVFDRQ
FRLTTANRGAERIFRQQFQEVLGSALEQIGVLSEFGGMVRKAFADREAASGDGHDDGGHW
QQQFSVQVAGESEPLTLLVRGARLVSATDRDAQDMQTSGYVVVFDDISDVISAQRSIAWG
EVARRLAHEIKNPLTPIQLSAERLQMKLADKLTPSDADVLKRGATTIVNQVAAMKQMVDN
FRDYARTPPAVLAHLQLNELVSEVLTLYGIEEGKGAIQVELSELPAIRGDATQLRQVIHN
LLQNAQDAVADVERPRVLLETRTVEYGDPDAQGKARIAVRLTVSDNGPGFPTRILTRAFE
PYVTTKAKGTGLGLAMVKKIVDEHGARIDIRNRMKAGDVIEGAQISILFLQLADDAAAPE
AGPRPAHGGTSQGKTKATVQTKAA