Protein Info for ABIE53_000311 in Paraburkholderia graminis OAS925

Annotation: DHA2 family multidrug resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 transmembrane" amino acids 34 to 55 (22 residues), see Phobius details amino acids 76 to 95 (20 residues), see Phobius details amino acids 104 to 123 (20 residues), see Phobius details amino acids 129 to 150 (22 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 191 to 212 (22 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 255 to 274 (20 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details amino acids 324 to 343 (20 residues), see Phobius details amino acids 355 to 374 (20 residues), see Phobius details amino acids 391 to 411 (21 residues), see Phobius details amino acids 425 to 443 (19 residues), see Phobius details amino acids 502 to 520 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 37 to 518 (482 residues), 533.4 bits, see alignment E=2.8e-164 PF07690: MFS_1" amino acids 42 to 436 (395 residues), 191.6 bits, see alignment E=1.9e-60 PF00083: Sugar_tr" amino acids 59 to 210 (152 residues), 37.5 bits, see alignment E=1.5e-13

Best Hits

Swiss-Prot: 52% identical to EMRB_ECO57: Multidrug export protein EmrB (emrB) from Escherichia coli O157:H7

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 96% identity to bug:BC1001_3576)

MetaCyc: 52% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (532 amino acids)

>ABIE53_000311 DHA2 family multidrug resistance protein (Paraburkholderia graminis OAS925)
MAATASNADEPAAQPAHQPATPPAAEPAPLKGGALALLTVGLALGTFMEVLDTSIANVAV
PTISGSLGVATSQGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLLFTIASALCG
FAHNFESLILFRLVQGLVSGPMVPLSQTILMRSYPPEKRGLALGLWAMTVICAPIFGPVM
GGYITDNYTWPWIFYINVPIGLFSAACAFVLLRGRETKTTQQRIDAIGLSLLVIGVACLQ
MVLDLGKDRDWFNSTFIVTLAVIAVISIAFMLVWEMTEKEPVVDLSLFKDRNFALGVLII
SFGFMAFFGSVVIFPLWLQTVMGYTAGLAGLATAPVGILALFLSPMIGKNMHRLNLRVIA
SFAFIVFAFVSFWNSTFTLDVPFNHVIWPRLVQGIGVACFFVPMTTITLSSVSDDRLASA
SGLSNFFRTLSGAIGTAISTTYWENDTIRHHAMLVDNVNVYTANTNAYTSALAGAGLSGD
SITAQLNQVVTAQAYMLATNDFFRISCAGFLVLAVLVWITKPRKGVGPSMGH