Protein Info for ABIE53_000067 in Paraburkholderia graminis OAS925

Annotation: choline dehydrogenase-like flavoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 PF00732: GMC_oxred_N" amino acids 3 to 295 (293 residues), 178.2 bits, see alignment E=4.2e-56 PF05199: GMC_oxred_C" amino acids 387 to 522 (136 residues), 144 bits, see alignment E=9.7e-46

Best Hits

Swiss-Prot: 47% identical to ALKJ_PSEPU: Alcohol dehydrogenase [acceptor] (alkJ) from Pseudomonas putida

KEGG orthology group: None (inferred from 93% identity to bug:BC1001_3363)

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (552 amino acids)

>ABIE53_000067 choline dehydrogenase-like flavoprotein (Paraburkholderia graminis OAS925)
MEYDYIIVGAGSGGCALASRLADNCPDATIALIEAGPHTNRNLLVNMPVGVAAVVPNKLK
TNYGYLTTPQPGLGGRRGYQPRGRGFGGSSAINAMIYTRGHPLDYDEWAQLGCDGWSWQD
VLPYFRRAEGNERGADAWHGDSGPLSVSDLRYRNPFSKRFVQAAMEAGYKPNDDFNGADQ
EGIGFYQVTQRDGRRCSVARAYIYDRERANLHTIADATVLRVVFDGKRASGVDVVRGGRR
ETLTARAEIVLAAGAFNSPQLLMCSGIGPAGHLRAHGIEVLHDAPEVGQNLIDHVDFTIN
KRVSSIEPTGFSVRGIARMVPQFVTFMRHGRGMLSSNVAEAGGFLKSRPTLERPDLQLHF
CAALVDDHNRHMHWGHGYSLHVCVLRPFSRGSVTLASADARDAPVIDPRFFSDSRDLDLL
VEGVQMARRILDAPSLALHGGRELYTRPGQSDEQLRQTIAEHADTIYHPVATCRMGGDAR
SVVDPQLRVRGVEGLRIVDASVMPTLIGGNTNSPTVMIGERAAELMTASRRRPQTVEQAG
AVASAAPTASAA