Protein Info for ABIE51_RS19215 in Lysobacter sp. OAE881

Annotation: bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1073 PF18327: PRODH" amino acids 33 to 77 (45 residues), 41.9 bits, see alignment (E = 1.9e-14) PF14850: Pro_dh-DNA_bdg" amino acids 86 to 199 (114 residues), 156.1 bits, see alignment E=9.1e-50 PF01619: Pro_dh" amino acids 209 to 508 (300 residues), 340.4 bits, see alignment E=1.9e-105 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 548 to 1051 (504 residues), 713.4 bits, see alignment E=6.7e-219 PF00171: Aldedh" amino acids 596 to 1055 (460 residues), 387.5 bits, see alignment E=1.6e-119

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 80% identity to xcv:XCV4008)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1073 amino acids)

>ABIE51_RS19215 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA (Lysobacter sp. OAE881)
MTIHSAHAVASDGPPAPHALISPELPPPPAAPRAAITAGWVRDEASHVRALLDRARLPEA
DRIAAQATAADLVRRVRARAKDQGAIEAFMRQYDLGSEEGVLLMCVAEALLRIPDQETAD
KLIRDKLGDADWKKHMGQSDSVLVNASTWGLMLTGHLVDLADETKRDVHGAFKRLLGRVG
EPVIRLAVRQAMKIMGHQFVMGRTIGEALARSQKNGNAAYRYSFDMLGEGALTTKDALRY
LQAYRDAIHAIGKSGDFKGKDVFAAPSISVKLSALHPRYEHAKRARVMSELVPRVLELAQ
MARSYGIGFTVDAEEADRLELSLDVIAAAYSDASLDGWEGYGLAIQAYQKRAPEAIDFIA
DLARRTGRRIPVRLVKGAYWDSEVKRAQVEGQPGYPVFTRKPNTDVSYLANARRMLEASD
AIYPMFATHNAQTIATIHQRAKSMGRAKHFEFQKLHGMGDDLYAEVIPADRLDVPCRVYA
PVGSHEDLLPYLVRRLLENGANSSFVNRITDEDIAIDDLVQDPVATVSAFESIAHPRIPQ
PLDLYRSFGIDRTNSMGINLANDDQLRTLAEQVNAAARSEWRATPLVPGANPAGPDIAVT
NPADRRQVVGQWKAADSATVEKAVQNAVAAHDAWNMTPAASRAAILEHAADLLEQRMPQY
IALCTREAGKSIPDGVAEVREAVDFLRYYAAQARKQFAIEPLPGPTGESNTLQLSGRGVF
VCISPWNFPLAIFLGQVAAALAAGNSVLAKPAEQTNLIGYYAVKLLREAGVPEAVVQFLP
GDGATVGAALTKDPRIAGVAFTGSTDTARAINRALAARDAAIGVLIAETGGQNALIADSS
SLPEQVVKDAIASAFTSAGQRCSAARVLFVQDDIADKVTTMLAGAMAELKVGDPGLLSTD
VGPVIDEDALKMLRDHAERMDREATKVAEVALDADAANGSFFAPRAYTLKSLDQLHKEIF
GPVLHVIRWKADQLDAVIDQINATGYGLTLGIHSRIDETIEKIASRIKVGNCYVNRNQIG
AVVGVQPFGGQNLSGTGPKAGGPHYLPRFATEKTITVNTTAAGGNASLLTLGD