Protein Info for ABIE51_RS19140 in Lysobacter sp. OAE881

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 TIGR01391: DNA primase" amino acids 3 to 412 (410 residues), 457.8 bits, see alignment E=1.6e-141 PF01807: zf-CHC2" amino acids 5 to 99 (95 residues), 130.3 bits, see alignment E=8.1e-42 PF08275: DNAG_N" amino acids 122 to 246 (125 residues), 138.1 bits, see alignment E=7.1e-44 PF13662: Toprim_4" amino acids 255 to 321 (67 residues), 62.3 bits, see alignment E=1.6e-20 PF01751: Toprim" amino acids 256 to 326 (71 residues), 50.3 bits, see alignment E=9.3e-17 PF13362: Toprim_3" amino acids 256 to 351 (96 residues), 34.3 bits, see alignment E=1.1e-11 PF13155: Toprim_2" amino acids 257 to 344 (88 residues), 60.6 bits, see alignment E=6.1e-20 PF10410: DnaB_bind" amino acids 364 to 417 (54 residues), 48.4 bits, see alignment 3.5e-16 PF08278: DnaG_DnaB_bind" amino acids 447 to 581 (135 residues), 82.2 bits, see alignment E=1.7e-26

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 72% identity to xal:XALc_0413)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (590 amino acids)

>ABIE51_RS19140 DNA primase (Lysobacter sp. OAE881)
MARIPDAFIDDLLARTDLVEVIGSRVQLKRQGKEYSARCPFHDERSPSFTVSPVKGFYHC
FGCGAHGTAISFLMNYDRLEFLDAVEELAKRLGMEVPRDTHQRNANPDSQDLYGALDAAS
KFFQKQLANNGRALGYFDSRGVDAETRARFSLGYAPDGFNALRDALGTDPRRMKLLERGG
LFSKNDRGHVYDKFRDRVMFPIHDRRGRTIAFGGRVLEKENGPKYLNSPETELFHKGREL
YGLWQVRQANSRIERLIVVEGYMDVIALFQNGFETAVATLGTATTPDHAELLFRNAPDVY
FCFDGDRAGRGAAWKAVESVLPRMKDGRQAFFLFLPDGEDPDSLVRNEGREGFEAHLKQA
TPLSQFFFDSLSSDVNLHTLEGKGRLAERAKPLLAQIPDGAFGDLMKQRLTELTGVGARA
STPETHVPAQRARAAGNAPHGSKPSLVRSAITMLMQQPQVALALPASLHFASLDQPGIGL
LVEIIELVHQRPDISTGMVLEHFADRSEAPALTKLATAGAVETTASTPLDARVLDDADRR
QQAFLDAIARLDAQALQARIESLQARMTSLVDAEKQELRELLSLRMLSRH