Protein Info for ABIE51_RS17520 in Lysobacter sp. OAE881

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 845 PF00696: AA_kinase" amino acids 16 to 292 (277 residues), 130.4 bits, see alignment E=2.3e-41 TIGR00657: aspartate kinase" amino acids 16 to 467 (452 residues), 324.8 bits, see alignment E=5.2e-101 PF13840: ACT_7" amino acids 321 to 379 (59 residues), 29.7 bits, see alignment 1.1e-10 PF03447: NAD_binding_3" amino acids 480 to 616 (137 residues), 53.8 bits, see alignment E=7.6e-18 PF00742: Homoserine_dh" amino acids 624 to 820 (197 residues), 152.9 bits, see alignment E=2e-48

Best Hits

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 64% identity to psu:Psesu_1249)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (845 amino acids)

>ABIE51_RS17520 bifunctional aspartate kinase/homoserine dehydrogenase I (Lysobacter sp. OAE881)
MPPADASRPSAPPCHVHKFGGSSLADASLYRAAAALVDDPSRTRVVVVSAMQGVTDALIA
LLETAQRRDAWHPAFHALRERHANAATALDPDGHFALQPALDAEFETLRRHLDAIGAGKA
DAGIFAADLPGFGEVFSSHLMHAALGGDAASWRRLDARDVLVVHAGEMGMAVDWPESRAR
LAKWRAGNPEGNVVITGFVARDAQGHATTLGRNGSDYSAAIFANLFDADALTIWTDVDGV
LSADPRLVPEAVCLPSMSYAEACELAYFGAKVLHPQTMAPVQERGIPLWIRNTKRPEAPG
TLIAPDSGRDGTPVKGLSLVRDLAIVELVGNGMVGVPGAAERLFGALRGAGVSVTMISQG
SSEHSICCVLRADQAERARDAAAAAFADAIADGQAQGVTLTRDIAVLAAVGDGMVGTPGV
AARLLGGLAQARVNARAIAQGAGERNISVAIGANDATRALRAAHAAFWLSPQTLSVGVIG
PGQVGTALLVQMSAALPQLRRRSGLDLRLRAIANSRAMVLDERGVEPADAAARLEGGAAQ
PVDLERFADHVRGEHLPHALIVDCSASPRVAAQYPRWLARGIHVVTPNKQAGSGAWDHYA
AIQDASRRGGGRFRYEATVGAGLPVMLTLRNLLDTGDELHGIDGMLSGTLAWLFNRFDGT
VPFSQLVREAQALGYTEPDPRDDLSGLDVARKLVILAREAGRALSLEDVDVENLVPPALR
EVGREEFLARLGEMDEPMLARQRDAARDGRSLRHLAQLDRDGVARVGVVALPAEHACCHT
RLTDNIVQFRTRRYSENPLVVQGPGAGPDVTAAGVFGDVLAIAQSLGVSSTWSSPPVEAR
VEVNA