Protein Info for ABIE51_RS17450 in Lysobacter sp. OAE881

Annotation: Mur ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 311 to 332 (22 residues), see Phobius details PF01225: Mur_ligase" amino acids 3 to 98 (96 residues), 61.9 bits, see alignment E=9.5e-21 PF08245: Mur_ligase_M" amino acids 109 to 318 (210 residues), 77.4 bits, see alignment E=2.3e-25 PF02875: Mur_ligase_C" amino acids 338 to 401 (64 residues), 41.8 bits, see alignment E=1.7e-14

Best Hits

KEGG orthology group: K02558, UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase [EC: 6.3.2.-] (inferred from 78% identity to psu:Psesu_2664)

Predicted SEED Role

"UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (EC 6.3.2.-)" (EC 6.3.2.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.-

Use Curated BLAST to search for 6.3.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>ABIE51_RS17450 Mur ligase family protein (Lysobacter sp. OAE881)
MLKIHILGIAGTFMGGVAALARELGHDVEGSDQAVYPPMSTQLENIGIALKQGYTPDHIS
ADCEQIVVGNALSRGNAAVEQVLDDGRRYTSGAQWLSENLLPGRDTLAVAGTHGKTTTTT
ILTYLLQAAGREPGFLIGGVAEDFGVSARVGQGREFVVEADEYDTAFFDKRSKFVHYRPL
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPRRGRLIVNGEDAHLAEVLAMGCWTPVE
TFGLDASQIPASAGERSHGDRADDSSVARAAFDWTARLIEQDGSRFAVVHRGVELGEVTW
PLLGRHNVMNALAALAAANAVGVDVAGVLGALSEFRSVKRRLEVIGQAQGVTIYDDFAHH
PTAIATTLAGLRAKVGDARIVVAMEPRSNSMRLGAHSDALAPSLDLADTVVFLHRPELAW
DAGKVVSALRHEGVAVPDADALIAALRERVRDGDHVVFMSNGGFDGAPRRFFAVLKDAAA
R