Protein Info for ABIE51_RS17295 in Lysobacter sp. OAE881
Annotation: RdgB/HAM1 family non-canonical purine NTP pyrophosphatase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 64% identical to IXTPA_XANC8: dITP/XTP pyrophosphatase (XC_0962) from Xanthomonas campestris pv. campestris (strain 8004)
KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 70% identity to xal:XALc_0794)MetaCyc: 57% identical to dITP/XTP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]
Predicted SEED Role
"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- dZTP biosynthesis (4/5 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
- pyrimidine deoxyribonucleotides dephosphorylation (2/3 steps found)
- tunicamycin biosynthesis (2/9 steps found)
KEGG Metabolic Maps
- Nicotinate and nicotinamide metabolism
- Pantothenate and CoA biosynthesis
- Purine metabolism
- Riboflavin metabolism
- Starch and sucrose metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.6.1.9
Use Curated BLAST to search for 3.6.1.15 or 3.6.1.66 or 3.6.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (200 amino acids)
>ABIE51_RS17295 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (Lysobacter sp. OAE881) MSARERWVIASSNQGKLREIRELLGDTGIEIVTQGELGVADADETGLTFVENALLKARKA SRETGLPALGDDSGLCVDALGGAPGLYSARYAGEHGNADANIDKLLHELRDVPDAQRTAH FYAVIVLLRHAEDPQPIVAEGLWRGRVLHERRGAGGFGYDPVFLDVEQGKSAAELDKDFK NRISHRGLALAALKRQLALL