Protein Info for ABIE51_RS17265 in Lysobacter sp. OAE881

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 730 TIGR00691: RelA/SpoT family protein" amino acids 48 to 727 (680 residues), 755.3 bits, see alignment E=3e-231 PF13328: HD_4" amino acids 48 to 197 (150 residues), 167.2 bits, see alignment E=8.3e-53 PF01966: HD" amino acids 67 to 166 (100 residues), 45.3 bits, see alignment E=3.6e-15 PF04607: RelA_SpoT" amino acids 257 to 367 (111 residues), 137.3 bits, see alignment E=9e-44 PF02824: TGS" amino acids 410 to 469 (60 residues), 68.5 bits, see alignment 1.5e-22 PF19296: RelA_AH_RIS" amino acids 481 to 567 (87 residues), 30.8 bits, see alignment E=1.1e-10 amino acids 586 to 642 (57 residues), 24.6 bits, see alignment 8.6e-09 PF13291: ACT_4" amino acids 653 to 729 (77 residues), 59 bits, see alignment E=2.1e-19 PF01842: ACT" amino acids 659 to 722 (64 residues), 30.3 bits, see alignment E=1e-10

Best Hits

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (730 amino acids)

>ABIE51_RS17265 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Lysobacter sp. OAE881)
MTPAQPAPTAPLPVPPLHDDAQVPDYVRELEQAADYLPDDQRAQLRRAWAVGAAAHVGQT
RKSGEPYITHPVAVAKVLADQGLDVETLIAAILHDTIEDTPLTYDGLVSEFGETVAELVD
GVTKLDKIQFRDRQEAAAESFRKMLLAMSRDLRVILIKLADRLHNMRTLGAQSMEARHRI
ARETLEIYAPIAQRLGMNLIKAELQDLGFRALHPWRHAVIEKRLRTQPVVRRESLVQIEA
QLAQRLAKEKLAHRLVSRVKSPWSIYTKMRAEHKTFAQVMDVFGFRIVVRTVPDCYHALG
VVHAAYKPLDSRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGIAAH
WSYKHGGDGPNSAQTRAHSWIANLVESQRATGSSLEFLENVKVDLFPDEVYLFTPKGDIM
SLPRNSTALDFAYAVHTDVGNRAVAARVDGRLVPLRTKLASGQRVEIITAKSSTPKPQWL
EFVVSGKARTSIRQQLKQLEHEDAVQLGHRMLDRALEALGISLDRVPAVRMDAYLNEFRY
PRLEALLADIALGNRMPQQVAMALAREAPGKPLRTPGGNMLPMSQDKILITGAERGVISF
ANCCLPIPGDEIMGYHTAGKGIVVHRLDCPNVADYRKSPDRWVPIGWDRQVAGDFSAALR
IEVDNRPGSLAQVAAAIAEAESNIDRVEYLERDANIAVMRFAIEVSDRRHLADVMRRVRR
LAVVLGVQRM