Protein Info for ABIE51_RS17230 in Lysobacter sp. OAE881

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 PF17191: RecG_wedge" amino acids 17 to 177 (161 residues), 50.4 bits, see alignment E=5.8e-17 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 35 to 675 (641 residues), 732.8 bits, see alignment E=2e-224 PF01336: tRNA_anti-codon" amino acids 68 to 142 (75 residues), 33.4 bits, see alignment E=1.1e-11 PF04851: ResIII" amino acids 278 to 443 (166 residues), 27 bits, see alignment E=1.2e-09 PF00270: DEAD" amino acids 280 to 447 (168 residues), 76.5 bits, see alignment E=6.6e-25 PF00271: Helicase_C" amino acids 493 to 599 (107 residues), 66.5 bits, see alignment E=7.2e-22 PF19833: RecG_dom3_C" amino acids 628 to 687 (60 residues), 51.9 bits, see alignment 2e-17

Best Hits

Swiss-Prot: 57% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 77% identity to sml:Smlt3838)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (705 amino acids)

>ABIE51_RS17230 ATP-dependent DNA helicase RecG (Lysobacter sp. OAE881)
MPIRKGGASVDLQQQPISALKGVGPRVAEKLAARGLATLQDLWLQLPRQYEDRTALTPIR
LLQPGVAVQVEGRVEAVERGFRYRPMLRVALGDDSHGTLVLRFFHFRAAQVAQFQVGARV
RCYGTPRPGQHGLEIVHPSYRMIDEAGDDALGTSLDPVYPAIEGLGPVSLRRLIGQALDR
LPDEDALELLPRELRDGSNLPSLREALLTVHRPPQDADVAALLAGRHPAQRRLAIEELLA
HHLSLRRQRIAQQAHAAPVLDRITLADTLRKRLPFALTGAQQRVFEQIRQDLAQPHPMLR
LVQGDVGSGKTVVAALAAMCAVEAGRQAALMAPTELLAEQHLNNLRGWLEPLGIRVAWLA
GKVTGKARAKVLEEVASGEAQVVVGTHALMQEGVAFNDLALAIVDEQHRFGVHQRLALRD
KGGGGSGNGDAIVPHQLVMTATPIPRTLAMAAYADLDVSAIDELPPGRTPVQTVALSAER
RPELVERIRKACAEGRQAYWVCTLIDDSDEVVAQAAQSTYEDLCKRLPEVRVGLVHGRMK
ATEKQATMRAFKDGEIGLLVATTVIEVGVDVPNASLMIIENAERLGLAQLHQLRGRVGRG
SAASSCVLLYQAPLSQMARQRLETMRETNDGFVIAEKDLELRGPGELLGTRQTGLAQFRV
ADLARDADLLPQVHEMGEKLLRESPELAERIVARWVGGAVRFASA