Protein Info for ABIE51_RS15245 in Lysobacter sp. OAE881

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 794 (794 residues), 741.9 bits, see alignment E=4.8e-227 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 79.4 bits, see alignment E=4.3e-26 PF03483: B3_4" amino acids 212 to 389 (178 residues), 211 bits, see alignment E=2.5e-66 PF03484: B5" amino acids 410 to 475 (66 residues), 73.7 bits, see alignment E=2.8e-24 PF17759: tRNA_synthFbeta" amino acids 478 to 686 (209 residues), 204.9 bits, see alignment E=2.8e-64 PF03147: FDX-ACB" amino acids 701 to 794 (94 residues), 98.3 bits, see alignment E=7.2e-32

Best Hits

Swiss-Prot: 69% identical to SYFB_XANC8: Phenylalanine--tRNA ligase beta subunit (pheT) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 73% identity to psu:Psesu_1120)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (795 amino acids)

>ABIE51_RS15245 phenylalanine--tRNA ligase subunit beta (Lysobacter sp. OAE881)
MKFSENWLRHHVPTTATRDELAATLTAIGLEVEEVTPLGDSLDGVVVARILSATRHPEAD
RLQVCEVDTGNGTVQIVCGAPNARAGLVAPLATVGATLPGGIAIKAAKLRGVESFGMLCS
AKELGVDPDASGLLELPDDAPVGTPLAQYLGLPDASIEIKLTPNRADCFSVRGIAFDVAA
AHGGEVKPLDAAPVAAGSDAAMRVELDAGPRVPRFAGRVIDGVDATVSTPAWMAERLRRS
GVRPISFLVDVTQYVMLELGQPMHAFDKDTLEGDVVVRPARAGEALKLLDGRTVELDDDF
LVVSDSRGGKAARAVALGGIMGGFDTRVTDATRNVFLEAAHWIPSAIIGRSRKLGLHTDA
GHRFERGVDPELPRIAVEYATKLILEIAGGVPGPTVDVTLAEHLRAPQPIVLRRARLARV
LGLSVADAEVERILRALGLQVESLADGWRVVPPSRRFDLEIEEDLIEEIARIHGYDAIPT
TLPAGAARLVAPSETRVDESTARRHLASRDFLEAVNYAFVDADLLSKWSLTDGSVPLANP
LSGELGVMRTALLPGLVAALARNAARQQPRVRLFELGNVFRDNPGEAPIETGRIAAAACG
DVHAEQWGIAARPIGFHDLKGDLDSLAALAGATLEYRASQPAWAHPGRSADVYRDGVHLG
WIGQLHPRLQRALELDVEVVAFELDLAPLFSRAVPRAAALSKYPSVRRDLAFVVPESVEW
AAIERAVKAAAGPFLRDLVLFDRYQGKGVEPGFKSLAMGLILQDESRTLTDRDVDTAVAS
VTGALQREYGATIRG