Protein Info for ABIE51_RS13425 in Lysobacter sp. OAE881

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR00435: cysteine--tRNA ligase" amino acids 3 to 454 (452 residues), 473.4 bits, see alignment E=4.9e-146 PF01406: tRNA-synt_1e" amino acids 16 to 313 (298 residues), 376.1 bits, see alignment E=2.2e-116 PF09190: DALR_2" amino acids 340 to 398 (59 residues), 45.7 bits, see alignment E=1.2e-15

Best Hits

Swiss-Prot: 78% identical to SYC_XANCP: Cysteine--tRNA ligase (cysS) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 80% identity to sml:Smlt3305)

MetaCyc: 46% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>ABIE51_RS13425 cysteine--tRNA ligase (Lysobacter sp. OAE881)
MSLHLFNSLTRRVEPFAPLNPDRPTMYLCGPTVYNFVHVGNARGPVVFGVLADVLRRRYG
SLDYARNITDIDDKINNAARERGVPIGEITARFTAAYLDDMAALGVKPQNIQPEATAHVP
QIIEMIQRLIDAGHAYEAEGHVLFAVGTFAGYGKLSRRDPEELLAGARIDVAPYKRDAGD
FVLWKPSTDDLPGWESPWGRGRPGWHIECSAMAAAHLGETIDIHAGGVDLQFPHHENEIA
QSECAHGGRIFARYWLHNGMLNFGGAKMAKSVGNIQRVHDLVQQYPPEALRFSLLSAHYR
QPLEWSDGLIEQSVRTLDRLYGTLRDLADVDAEPAIPATVEAALDDDLNTPQVMAEIARI
AGDARKAVSADEKARLKSELLGAGLALGLLQQDPAAWFARGTEEGDDARIQALIDERLAA
KKARDFARADAIRDQLAGEGILLEDTPQGVRWKRA