Protein Info for ABIE51_RS13370 in Lysobacter sp. OAE881

Annotation: glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 16 to 368 (353 residues), 364.6 bits, E=2.9e-113
• PF00696: AA_kinase
  • amino acids 16 to 232 (217 residues), 123 bits, E=1.7e-39
• PF01472: PUA
  • amino acids 279 to 353 (75 residues), 68.1 bits, E=5.1e-23

Best Hits

Swiss-Prot: 62% identical to PROB_XYLFA: Glutamate 5-kinase (proB) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 65% identity to xoo:XOO2666)

MetaCyc: 45% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (380 amino acids)

>ABIE51_RS13370 glutamate 5-kinase (Lysobacter sp. OAE881)
MNGSDFTAQPLPPWRRAVLKVGSSLLSGEDGLDPRHAGALARFIEHARTQQREIVLVSSG
AVAAGRHRIAAGGENLVLRQALASLGQAALVTFWQQLVDRPVAQVLLTHDDLRNRRRYLN
ARATLRELLRLGALPVINENDTVAVDELKLGDNDNLAAAVASLVDADLLLIATDIGGLYS
AHPRDPAAQAIMRVETITPELLQAASGGAGTLGTGGMRTKLDAAAKAGAAGIATALFCGR
DADVVRALHDDVLHGTLVLAQGDRLRARKQWLRHAPSSGRLHVDAGAFAALHRQGASLLP
GGVVSAKGEFRRGDVVEIGVEHEPAFARGLAQYGANEVRRIARRHSNEIEAILGFRYGES
VVHRDDLVLIDATSHKETST