Protein Info for ABIE51_RS13160 in Lysobacter sp. OAE881
Annotation: carboxyl transferase domain-containing protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to MCCB_MOUSE: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Mccc2) from Mus musculus
KEGG orthology group: K01969, 3-methylcrotonyl-CoA carboxylase beta subunit [EC: 6.4.1.4] (inferred from 86% identity to psu:Psesu_1640)MetaCyc: 68% identical to propionyl-CoA carboxylase alpha subunit (Chloroflexus aurantiacus)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]
Predicted SEED Role
"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)
MetaCyc Pathways
- superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
- L-leucine degradation I (5/6 steps found)
- propanoyl CoA degradation I (2/3 steps found)
- 2-oxobutanoate degradation I (2/4 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
- 3-hydroxypropanoate cycle (8/13 steps found)
- 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
- methylaspartate cycle (11/19 steps found)
- superpathway of L-methionine salvage and degradation (8/16 steps found)
- superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
- mycolate biosynthesis (25/205 steps found)
- superpathway of mycolate biosynthesis (26/239 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.4.1.4
Use Curated BLAST to search for 6.4.1.3 or 6.4.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (541 amino acids)
>ABIE51_RS13160 carboxyl transferase domain-containing protein (Lysobacter sp. OAE881) MPALTSHIDPRSQEFQDNVAYHRALVDELDARLARAAGGGGEKARAKHTERGKLLARDRI AALLDPGSPFLEIAPLAAEGMYDDAAPAAGMVCGIGRVMGQEVVIVANDATVKGGTYFPM TVKKHLRAQEIARENRLPCVYLVDSGGAFLPLQDEVFPDKEHFGRIFYNQARLSAENIPQ VAVVMGSCTAGGAYVPAMCDESIIVKEQGTIFLGGPPLVKAATGEVVDAEALGGADVHTS VSGVADHFAEDDRHALQIARDIVGTFNRRKTLPVAVRESREPSFPTGELYGIVPKDTRRP FDIREVIARVVDGSEFQEFKARYGKTLVTGFAHVHGYPVGIVANNGILFAESALKGAHFI ELCNQRGIPLVFLQNITGFMVGRKYENAGIAKDGAKMVTAVACSHVPKFTVVIGGSFGAG NYAMCGRAYGARFLWMWPNARISVMGGEQAASVLATVKRDGIEAAGKSWSSEEEDAFKAP IREQYESQGNPYYATARLWDDGIIDPADTRRVLGLALSASFNAPIESAPGDASTRFGVFR M