Protein Info for ABIE51_RS12630 in Lysobacter sp. OAE881

Annotation: asparagine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 TIGR00457: asparagine--tRNA ligase" amino acids 13 to 466 (454 residues), 639.2 bits, see alignment E=1.8e-196 PF01336: tRNA_anti-codon" amino acids 20 to 100 (81 residues), 50 bits, see alignment E=2.2e-17 PF00152: tRNA-synt_2" amino acids 125 to 459 (335 residues), 223.8 bits, see alignment E=3.1e-70

Best Hits

Swiss-Prot: 85% identical to SYN_XYLFT: Asparagine--tRNA ligase (asnS) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K01893, asparaginyl-tRNA synthetase [EC: 6.1.1.22] (inferred from 89% identity to xal:XALc_1738)

MetaCyc: 68% identical to asparagine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Asparagine--tRNA ligase. [EC: 6.1.1.22]

Predicted SEED Role

"Asparaginyl-tRNA synthetase (EC 6.1.1.22)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (466 amino acids)

>ABIE51_RS12630 asparagine--tRNA ligase (Lysobacter sp. OAE881)
MTVVSVEQALAGKIPAGGEVTVRGWVRTRRDSKAGLSFVNVSDGSCFAPIQVVAPAELPN
YESEVKRLTAGCAVIATGTLVASQGQGQAFEIQASSLEVVGWVEDPETYPIQPKAHSLEF
LREVAHLRPRTNLFGAVTRIRDCLAKAAHRYFHENGFYWISTPIVTTSDAEGAGQMFRVS
TLDMANLPRDDKGGIDFSRDFFGKETFLTVSGQLNVEAYALSLSKVYTFGPTFRAENSNT
TRHLAEFWMIEPEIAFADLAEDARVAEEFLKYMFRAVLAERADDMAFIAERVQKDAITRL
ESFVNAPFERIEYTDAIKLLQKADKKFDFPVEWGLDLQTEHERWLTEEHVGRPVVVTNYP
EHIKAFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREERLDMLDARMAQFGLDPAHYGWY
RDFRRYGTVPHAGFGLGFERLVVYVCGLSNIRDAIPYPRAPGSAEY