Protein Info for ABIE51_RS12545 in Lysobacter sp. OAE881

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 570 (569 residues), 474.6 bits, E=2.4e-146
• PF13522: GATase_6
  • amino acids 35 to 167 (133 residues), 165.3 bits, E=1.5e-52
• PF13537: GATase_7
  • amino acids 52 to 171 (120 residues), 159.3 bits, E=8.1e-51
• PF00733: Asn_synthase
  • amino acids 263 to 635 (373 residues), 377.1 bits, E=2.6e-116
• PF06508: QueC
  • amino acids 283 to 344 (62 residues), 23.6 bits, E=6.8e-09

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 74% identity to smt:Smal_2639)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-asparagine biosynthesis (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (647 amino acids)

>ABIE51_RS12545 asparagine synthase (glutamine-hydrolyzing) (Lysobacter sp. OAE881)
MCGLAGLLRPTADLDAARLDALAGAMGDALEHRGPDDRGVWVDDTTGIALAHRRLSILDL
SPLGHQPMASEDHRYVVVYNGEIYNFAELREVLVARGHVFRGHSDTEVLLAAVMEWGVED
AVSRCNGMFAIALWDRQERCLWLARDRVGKKPLYYGWAGGTFVFGSELKALWRHPDFDNG
VDPDALALLLRLDYIPAPHAIHVDTFKLLPGSLLRIDAATVAGGPRAHDPHRDQRRYWEP
RERMHAALAQPFNGDVLEAENRLDDLLRDAVSLRMVADVPVGVFLSGGTDSSIVTALMQT
QSRHPVRTFTIGFERSHHDEAHLAREVAEHLHTQHTELYVSGADALGVVPTLPAMFDEPF
ADASQVPTALVARLARTAVTVALSGDGGDELFFGYKRYERALRNWRWHSRVPGFLRHALA
GSAKTHGEASRTGGFAALAAEMGARGIGDVYRQRVMRWRDPVAVVPGAKLPDTFYDQPDP
LRGAGTPADAMMLADFSVYLPEDLLCKVDRTSMAVSLEARAPLLDWRVAEFAWSLPLDFK
RHPSGNKHLLKRVLRRYLPDSMVDRGKRGFGAPVSDWLKGDLREWAGALLDPARLRAEGH
LDADAVDALWQQFLGGERKWHTHLWNVLMFQAWHEHWQSQRSAVRAI