Protein Info for ABIE51_RS11625 in Lysobacter sp. OAE881

Annotation: glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 5 to 371 (367 residues), 479 bits, E=4.3e-148
• PF00988: CPSase_sm_chain
  • amino acids 7 to 131 (125 residues), 168 bits, E=1.3e-53
• PF00117: GATase
  • amino acids 192 to 368 (177 residues), 180.9 bits, E=3.3e-57
• PF07722: Peptidase_C26
  • amino acids 246 to 281 (36 residues), 21.5 bits, 2.6e-08

Best Hits

Swiss-Prot: 81% identical to CARA_XANCP: Carbamoyl-phosphate synthase small chain (carA) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 82% identity to xal:XALc_1338)

MetaCyc: 65% identical to carbamoyl-phosphate synthetase small subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline degradation (2/3 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-arginine biosynthesis IV (archaea) (3/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (374 amino acids)

>ABIE51_RS11625 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit (Lysobacter sp. OAE881)
MTQPAILALEDGTLFEGVSVGAPGLSVGEVVFNTAITGYQEIVTDPSYARQLVTLTYPHI
GNTGCTDQDDEAARCFASGLIVRNVPRRPSNWRSQVSLPEWLNARGIVAISDIDTRKLTR
ILREKGAQNGALMAGEIDVDKAFEAARKFPGLKGMDLAKEVSVRESYEWTDGQLDLDNNA
FVRAEPKFHVVAYDYGVKFNILRMLAERGCKLTVVPAQTPASDVFALKPDGVFLSNGPGD
PAPCDYAIAAIKEFISAKIPTFGICLGHQLLALAAGAQTVKMGHGHHGANHPVQDLDTGR
VMITSQNHGFAVDEATLPANVRVIHRSLFDGTNQGIELTDAPAFSFQGHPEASPGPHDVA
PLFDRFVTMMQQAK