Protein Info for ABIE51_RS11535 in Lysobacter sp. OAE881

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 19 to 915 (897 residues), 1349.9 bits, E=0
• PF00330: Aconitase
  • amino acids 71 to 584 (514 residues), 546.5 bits, E=6.3e-168
• PF00694: Aconitase_C
  • amino acids 714 to 845 (132 residues), 139.5 bits, E=8.4e-45

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99)" (EC 4.2.1.3, EC 4.2.1.99)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• 2-methylcitrate cycle II (4/6 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• methylaspartate cycle (11/19 steps found)
• reductive TCA cycle II (5/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.99

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Protein Sequence (921 amino acids)

>ABIE51_RS11535 aconitate hydratase AcnA (Lysobacter sp. OAE881)
MSQDSFQTRRTLDVGGRSLTYFSLPALGERFDIGRLPYSMKILLENLLRHEDGGVTVGPE
HIEAVAKWDCKAEPDTEIAFMPARVVLQDFTGVPCVVDLAAMRDAVTKLGGNPRQINPLI
PSELVIDHSVQVDVFGTPDALDLNGKIEFQRNMERYSFLRWGQKAFDNFKVVPPNTGIVH
QVNLENLARVVVERDVDGEQLAFPDTVFGTDSHTTMINGIGVLGWGVGGIEAEAAMLGQP
SSMLIPQVVGFKLTGALPEGTTATDLVLTVTQMLRKLGVVGKFVEFFGDGLQHLPLADRA
TIANMAPEYGATCGIFPIDAESLNYLRLSGRSDAQIALVEAYAKAQGLWHEPGAPEAAYS
TTLELDLGDVKPSLAGPKRPQDRVLLVDVKQNYRDNLGPLTAGRDKRSPDVANFVAEGGG
AAVGNESLHKGMADIRIKDENVRLKDGAVVIAAITSCTNTSNPAVMLGAGLLARNAAKLG
LKAKPWVKTSLGPGSLVVTDYLRKAGVLDDLEKLGFFVVGYGCTTCIGNSGPLPTEVSAG
IAAGDLVVASVLSGNRNFEGRVHPEVKMNYLASPPLVVAYAIAGTVDIDLSREPLGKGAD
GQDVFLRDIWPTNKEIGDVIAATVGPELFAQNYADVFKGDTRWNTIASPEGESFAWDEAS
TYIKNPPYFDGMSMQVGTIDDIHDARVLGLFGDSITTDHISPAGNIKKDSPAGRFLISRG
VQPADFNSYGSRRGNDDVMVRGTFANIRIKNLMFGGEEGGNTLYFGKDGAAPEKLSIYDA
AMKYKADGVPLVVLAGKEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLP
LLFKEGENAQTLGLDGSETFSVTGLQDGESKRATVTATRKDGSTVSFEVKVMLLTPKEVE
YFRHGGILHYVLRQLAAKKSL