Protein Info for ABIE51_RS10035 in Lysobacter sp. OAE881

Annotation: glyoxylate/hydroxypyruvate reductase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 105 to 276 (172 residues), 153.7 bits, E=3.4e-49
• PF03446: NAD_binding_2
  • amino acids 137 to 225 (89 residues), 26 bits, E=8.8e-10

Best Hits

Swiss-Prot: 43% identical to GHRA_YERE8: Glyoxylate/hydroxypyruvate reductase A (ghrA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K12972, gyoxylate/hydroxypyruvate reductase A [EC: 1.1.1.79 1.1.1.81] (inferred from 48% identity to bte:BTH_I0314)

MetaCyc: 41% identical to glyoxylate/hydroxypyruvate reductase A (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; 1.1.1.- [EC: 1.1.1.79]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• L-serine biosynthesis II (1/4 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.79, 1.1.1.81, 1.1.1.95

Use Curated BLAST to search for 1.1.1.79 or 1.1.1.81 or 1.1.1.95

Protein Sequence (311 amino acids)

>ABIE51_RS10035 glyoxylate/hydroxypyruvate reductase A (Lysobacter sp. OAE881)
MTTHDNPHVLICTAKDQDDWRDALAQCLPDATLHAGVEAPPCDYAVVWKPPHALFVRQTK
LKAIFSLGAGVNALVAMPSLPRDVPLVRMEDGGMADQLVEYALYVALRRLRGFTAYESDQ
QAQRWSPRPARSRHELRVGVLGLGALGEKVARALADFGFTVTGWSRTPKDIAGIATEHGP
DGLDRVLARSDLAVVLLPLTDATRGILDTERLRMLPAGASIANLSRGEVLDDAALLALLD
ANHIDEAHLDVFAQEPLPLDHPYWRHPRVHMTPHVAALTDPLLAAREVAEKIGRLETGLP
ITGVVDFERQY