Protein Info for ABIE51_RS09520 in Lysobacter sp. OAE881

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 788 (784 residues), 1200.6 bits, E=0
• PF01326: PPDK_N
  • amino acids 18 to 345 (328 residues), 411.4 bits, E=4.4e-127
• PF00391: PEP-utilizers
  • amino acids 383 to 454 (72 residues), 95 bits, E=2.7e-31
• PF02896: PEP-utilizers_C
  • amino acids 480 to 787 (308 residues), 206.3 bits, E=8.7e-65

Best Hits

Swiss-Prot: 67% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 87% identity to psu:Psesu_1439)

MetaCyc: 65% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Protein Sequence (794 amino acids)

>ABIE51_RS09520 phosphoenolpyruvate synthase (Lysobacter sp. OAE881)
MNENILWLHALRLTDLARVGGKNSSLGEMIGNLANLGVSVPGGYATTAEAFKDFIAHNDL
HQRIYDKLATLDVEDVNALTAAGAEIRGWVIDAPLQPELDADIRKAYKQLCDENGGGDVA
VAVRSSATAEDLPDASFAGQQETFLNVTGADDVVHKVKEVFASLYNDRAIAYRVHHGFKH
EDVFLSAGVQLMVRSNVGSSGVLFTLDTESGFRDVVFITSSYGLGEMVVQGAVNPDEFYV
YKPTLLQGKQAILRRGMGAKQLRMVYSDTPGERVRTEETPADLRNTFSISDEDVHELARQ
ALTIEKHYGRPMDIEWAKDGVSGKLFIVQARPETVKSRGHATQIERYQLTQRGEVVCEGR
AIGQKIGTGVARVVRSLADMNRVQPGDVLVADMTDPDWEPVMKRSAAIVTNRGGRTCHAA
IIARELGVPAVVGTGNALDTIQDGQEVTVSCAEGDTGFIYAGTLPFERHVADLDKMPEAP
LKVMMNVANPERAFDFAMLPHAGVGLARLEMIIASHIGVHPKALLEYDQQDAATKKKIDE
KMAGYASPVDFYVDRLAEGIATITASFAPYPVIVRLSDFKSNEYANLIGGSRYEPHEENP
MIGFRGASRYVDPSFTEAFALECRAVLKVREQMGLTNCWVMIPFVRTLEEGRKVVEVLRS
NGLEQGKDGLKIIMMCEVPSNALLADEFLEIFDGFSIGSNDLTQLTLGLDRDSGIVAKLF
DERDPAVKKLLSLAIKSARAKGKYVGICGQGPSDHPDLAEWLMDQGIESVSLNPDTVVDT
WLRLAKAKAKKAAA