Protein Info for ABIE51_RS09495 in Lysobacter sp. OAE881

Annotation: Fe/S-dependent 2-methylisocitrate dehydratase AcnD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 864 (863 residues), 1799.4 bits, E=0
• PF00330: Aconitase
  • amino acids 66 to 538 (473 residues), 455.9 bits, E=1.9e-140
• PF00694: Aconitase_C
  • amino acids 662 to 793 (132 residues), 98.6 bits, E=3.8e-32

Best Hits

Swiss-Prot: 89% identical to ACNA_CUPNE: Aconitate hydratase A (acnM) from Cupriavidus necator

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 88% identity to bcj:BCAM2701)

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• methylaspartate cycle (11/19 steps found)
• reductive TCA cycle II (5/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.79

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.79

Protein Sequence (870 amino acids)

>ABIE51_RS09495 Fe/S-dependent 2-methylisocitrate dehydratase AcnD (Lysobacter sp. OAE881)
MNSQHRKPLPGTSLEYFDAREAVEAIQPGAWAGLPYTSRVLAENLVRRCDPTTLVASLKQ
LIERRRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQGGDPAKVNPVVPVQLIVDH
SLAVECGGYDPDAFAKNRAIEDRRNEDRFHFIEWTKRAFENVDVIPPGNGIMHQINLEKM
SPVIQSQGGVAFPDTCVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGV
ELTGKRQPGITATDIVLALTEFLRKEKVVGAYLEFRGEGASSLTLGDRATISNMAPEYGA
TAAMFFIDNQALEYLRLTGRDDAQVKLVETYAKTAGLWADDLAHAQYERTLTFDLSSVVR
NMAGPSNPHKRLPTSDLAERGIAVNLDAALAQEAQGLMPDGAVIIAAITSCTNTSNPRNV
IAAALLARNANARGLTRKPWVKTSLAPGSKAVQLYLEDAKLLPELEKLGFGIVAFACTTC
NGMSGALDPAIQQEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTV
RFDIERDALGLDADGKPVTLKDIWPSDEEIDAIVAQSVKPEQFRRVYEPMFNIRVEHGER
VSPLYDWRPQSTYIRRPPYWEGALAGERTLRGLRPLAVLGDNITTDHLSPSNAILMNSAA
GEYLHKMGLPEEDFNSYATHRGDHLTAQRATFANPTLVNEMAVVNGEVKKGSLARIEPDG
QVVRMWEAIETYMERRQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTN
LIGMGVLPLEFKPGFNRTVLRIDGTETFDVTGERKPRADLTLVIHRRDGERVEVPVTCRL
DSDEEVSIYEAGGVLQRFAQDFLESSARAA